Alyousef Yousef M, Borgio J Francis, AbdulAzeez Sayed, Al-Masoud Naif, Al-Ali Ali A, Al-Shwaimi Emad, Al-Ali Amein K
College of Dentistry, Institute for Research and Medical Consultation (IRMC), University of Dammam, Dammam, Saudi Arabia.
Caries Res. 2017;51(1):12-16. doi: 10.1159/000450963. Epub 2016 Nov 29.
The high prevalence of dental caries in children worldwide is a major oral health problem which requires early intervention. Dental caries is mainly caused by the action of acids produced by bacteria in addition to many other factors. Recent genetic studies have reported that a number of genes are associated with the susceptibility to dental caries. The majority of these genes are associated with inflammation, increased susceptibility to infection, and dentine matrix formation. Using the TaqMan assay and direct DNA sequencing, the prevalence of 6 single-nucleotide polymorphisms (SNPs) in MMP9, MBL2, MMP2, and TIMP2 genes was determined in 102 children with caries and in 100 age-matched caries-free controls. Out of the 6 SNPs tested in the 4 selected genes, only rs11003125 in the MBL2 gene was shown to be associated with a high prevalence of caries in our cohort. In addition, haplotype analysis of the 6 SNPs tested revealed that certain haplotypes, namely GT of rs11003125G and rs7501477T and GT of rs7096206G and rs7501477T, were found to be associated with a high prevalence of dental caries in our cohort, while haplotype AG of rs17576A and rs7501477G was found to have a protective effect against dental caries. In conclusion, the data indicate that rs11003125 in the MBL2 gene was shown to be associated with a high prevalence of caries in our cohort, and 2 haplotypes are also involved in the increased susceptibility to dental caries.
全球儿童龋齿的高患病率是一个主要的口腔健康问题，需要早期干预。龋齿主要是由细菌产生的酸的作用引起的，此外还有许多其他因素。最近的基因研究报告称，一些基因与龋齿易感性有关。这些基因中的大多数与炎症、感染易感性增加和牙本质基质形成有关。使用TaqMan检测法和直接DNA测序法，在102名患龋儿童和100名年龄匹配的无龋对照儿童中测定了MMP9、MBL2、MMP2和TIMP2基因中6个单核苷酸多态性（SNP）的患病率。在所选的4个基因中检测的6个SNP中，只有MBL2基因中的rs11003125与我们队列中龋齿的高患病率相关。此外，对检测的6个SNP进行单倍型分析发现，某些单倍型，即rs11003125G和rs7501477T的GT以及rs7096206G和rs7501477T的GT，与我们队列中龋齿的高患病率相关，而rs17576A和rs7501477G的单倍型AG对龋齿有保护作用。总之，数据表明，MBL2基因中的rs11003125与我们队列中龋齿的高患病率相关，并且2种单倍型也与龋齿易感性增加有关。
