Shaker Olfat G, Abdelaleem Omayma O, Fouad Nermeen A, Ahmed Naglaa A, Hussein Hoda A, Ibrahem Enas G, Mohamed Abdelrahmaan A, Ahmed Othman M, Ali Doaa Y
Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt.
Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Fayoum University, Faiyum, Egypt.
Arch Med Sci. 2021 Mar 19;18(6):1467-1474. doi: 10.5114/aoms/105349. eCollection 2022.
The current study was designed to analyze whether polymorphisms of miR-146a and miR-155 are related to Behçet's disease (BD) in the Egyptian population.
A total of 96 unrelated BD patients and 100 healthy subjects were genotyped for miR-146a (rs2910164) and miR-155 (rs767649) using real-time polymerase chain reaction.
The results showed significant elevation in the frequency of rs2910164 GG and CC genotypes in BD patients compared with controls (adjusted OR = 22.156, 95% CI: 4.728-103.818; < 0.001 and adjusted OR = 40.358, 95% CI: 8.928-182.440; < 0.001, respectively). Also, the rs2910164 G allele conferred a higher risk of developing BD (adjusted OR = 3.665, 95% CI: 2.013-6.671; < 0.001). MiR-146a (rs2910164) polymorphism was a risk factor for susceptibility to BD in dominant, recessive and additive models of inheritance (all < 0.001), while the miR-155 (rs767649) polymorphism was a risk factor in the recessive model only ( = 0.021). GG and CG genotypes of rs2910164 were associated with higher Behcet's disease current activity index (BDCAI) and ocular involvement compared with CC genotype ( = 0.005 and = 0.004, respectively). Genotype AT of rs767649 was related to higher BDCAI ( = 0.026) compared with TT and AA genotypes.
miR-146a (rs2910164) and miR-155 (rs767649) are likely to play an important role in the Egyptian population in development of BD and also influence disease severity.
本研究旨在分析埃及人群中miR - 146a和miR - 155的多态性是否与白塞病（BD）相关。
采用实时聚合酶链反应对96例无亲缘关系的BD患者和100例健康受试者进行miR - 146a（rs2910164）和miR - 155（rs767649）基因分型。
结果显示，与对照组相比，BD患者中rs2910164的GG和CC基因型频率显著升高（校正OR = 22.156，95%CI：4.728 - 103.818；P < 0.001和校正OR = 40.358，95%CI：8.928 - 182.440；P < 0.001）。此外，rs2910164的G等位基因赋予了更高的患BD风险（校正OR = 3.665，95%CI：2.013 - 6.671；P < 0.001）。在显性、隐性和加性遗传模型中，miR - 146a（rs2910164）多态性是BD易感性的危险因素（均P < 0.001），而miR - 155（rs767649）多态性仅在隐性模型中是危险因素（P = 0.021）。与CC基因型相比，rs2910164的GG和CG基因型与更高的白塞病当前活动指数（BDCAI）和眼部受累相关（分别为P = 0.005和P = 0.004）。与TT和AA基因型相比，rs767649的AT基因型与更高的BDCAI相关（P = 0.026）。
miR - 146a（rs2910164）和miR - 155（rs767649）可能在埃及人群中BD的发生发展中起重要作用，并且还影响疾病严重程度。
