Chen Zhi-Cheng, Wu Shing-Yi Sean, Su Wei-Yang, Lin Yuan-Chuan, Lee Yi-Hsin, Wu Wei-Hao, Chen Chun-Hong, Wen Zhi-Hong
Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung, Taiwan.
Department of Chinese Medicine for Post-Baccalaureate, I-Shou University, Kaohsiung, Taiwan.
BMC Complement Altern Med. 2016 Nov 28;16(1):487. doi: 10.1186/s12906-016-1473-6.
The shell of Haliotis diversicolor, or shijueming (SJM), is a type of traditional Chinese medicine. The SJM has appeared in historical records as early as the third and fourth centuries. Historical records have revealed that SJM had mainly been used to treat eye diseases. After the Qing Dynasty (1757), records had emerged, detailing the use of SJM for treating skin injuries, particularly for treating poorly managed ulcers or traumatic wounds. Furthermore, in our anti-inflammation-screening system, SJM significantly inhibited the expression of pro-inflammatory proteins. Previous studies have yet to adopt an animal model to verify the phenomenon and described in the historical records regarding the efficacy of SJM in promoting wound healing. Besides, the mechanism of wound healing effect of SJM is also not clear.
This study applied in vitro and in vivo models, tissue section analysis, and western blotting to evaluate the effect of SJM on wound healing. The RAW 264.7 cells were used in anti-inflammatory activity assay and phagocytic assay. Male Wistar rats were used to evaluate the effect of SJM on burn injury healing. A copper block (2 × 2 cm, 150 g) preheated to 165 °C in a dry bath was used to contact the skin area for 10 s, thus creating a full-thickness burn injury. The results were analyzed by hematoxylin and eosin staining, picrosirius red staining and Western blotting.
The results revealed that in the in vitro model, the presence of SJM decreased the inducible nitric oxide synthase (iNOS) expression and enhanced the functions of macrophages. The results of the rat burn injury model revealed that SJM decreased neutrophil infiltration, promoted wound healing, thus increasing the collagen I content and promoting the expression of transforming growth factor-beta 1 (TGF-β1) protein. We speculate that the effect and mechanism of SJM on promoting wound healing is related to macrophage activation. In the inflammation phase, SJM alleviates inflammation by inhibiting iNOS expression and removing neutrophils through phagocytosis. Furthermore, SJM induces the secretion of TGF-β1, converting collagen during the tissue remodeling phase.
According to our review of relevant literature, this is the first study that applied an evidence-based method to verify that SJM alleviates inflammation, enhances phagocytosis, and triggers wound healing after burn injury. The study findings reveal that SJM provides a promising therapeutic option for treating burn injury.
杂色鲍的贝壳，即石决明（SJM），是一种传统中药。石决明早在公元三四世纪就已出现在历史记载中。历史记载表明，石决明主要用于治疗眼部疾病。清朝（1757年）以后，有记载详细描述了石决明用于治疗皮肤损伤，特别是治疗处理不当的溃疡或创伤性伤口。此外，在我们的抗炎筛选系统中，石决明显著抑制促炎蛋白的表达。以往的研究尚未采用动物模型来验证这一现象，也未描述历史记载中有关石决明促进伤口愈合功效的内容。此外，石决明促进伤口愈合的作用机制也不清楚。
本研究应用体外和体内模型、组织切片分析和蛋白质印迹法来评估石决明对伤口愈合的影响。RAW 264.7细胞用于抗炎活性测定和吞噬测定。雄性Wistar大鼠用于评估石决明对烧伤愈合的影响。将一个在干浴中预热至165°C的铜块（2×2厘米，150克）与皮肤区域接触10秒，从而造成全层烧伤。结果通过苏木精和伊红染色、天狼星红染色和蛋白质印迹法进行分析。
结果显示，在体外模型中，石决明的存在降低了诱导型一氧化氮合酶（iNOS）的表达并增强了巨噬细胞的功能。大鼠烧伤模型的结果表明，石决明减少了中性粒细胞浸润，促进了伤口愈合，从而增加了I型胶原蛋白含量并促进了转化生长因子-β1（TGF-β1）蛋白的表达。我们推测，石决明促进伤口愈合的作用及其机制与巨噬细胞激活有关。在炎症阶段，石决明通过抑制iNOS表达和通过吞噬作用清除中性粒细胞来减轻炎症。此外，石决明诱导TGF-β1的分泌，在组织重塑阶段转化胶原蛋白。
根据我们对相关文献的综述，这是第一项应用循证方法验证石决明可减轻炎症、增强吞噬作用并促进烧伤后伤口愈合的研究。研究结果表明，石决明为治疗烧伤提供了一种有前景
