生长和分化的Friend细胞中mRNA和前体mRNA的频率分布。

Frequency distribution of mRNA and pre-mRNA in growing and differentiated Friend cells.

作者信息

Mauron A, Spohr G

出版信息

Nucleic Acids Res. 1978 Aug;5(8):3013-32. doi: 10.1093/nar/5.8.3013.

DOI:10.1093/nar/5.8.3013

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342223/

Abstract

The frequency distribution of poly(A)+-mRNA in growing and in differentiated Friend cells has been measured by mRNA-cDNA hybridization and their differences established by heterologous hybridization of mRNA of one type and cDNA of the other. It was shown that induction of Friend cells involves an increase in abundance of a small number of mRNAs, while no specific pattern of messenger disappearance could be detected. The frequency distribution of pre-mRNA was determined by hybridizing nuclear RNA with the cDNA probes complementary to mRNA. In uninduced Friend cells, it was shown that most precursor messenger sequences are present at a single frequency of about 3 molecules per nucleus, independently of their final frequency in polysomal mRNA. In induced Friend cells, the frequency distribution of pre-mRNA is more heterogeneous and correlated to some extent with the corresponding mRNA frequency distribution.

摘要

通过mRNA-cDNA杂交测定了生长中的和分化的Friend细胞中多聚腺苷酸（poly(A)+）-mRNA的频率分布，并通过一种类型的mRNA与另一种类型的cDNA的异源杂交确定了它们之间的差异。结果表明，Friend细胞的诱导涉及少数mRNA丰度的增加，而未检测到信使消失的特定模式。通过将核RNA与与mRNA互补的cDNA探针杂交来确定前体mRNA的频率分布。在未诱导的Friend细胞中，结果表明大多数前体信使序列以每个细胞核约3个分子的单一频率存在，与它们在多核糖体mRNA中的最终频率无关。在诱导的Friend细胞中，前体mRNA的频率分布更不均匀，并且在一定程度上与相应的mRNA频率分布相关。

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Expression of single copy DNA sequences in nuclear RNA from undifferentiated mouse embryonal carcinoma and differentiated muscle cell line.未分化小鼠胚胎癌细胞和分化肌细胞系的核RNA中单一拷贝DNA序列的表达

Nucleic Acids Res. 1979 Apr;6(4):1639-55. doi: 10.1093/nar/6.4.1639.

本文引用的文献

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Erythrocytic maturation in vitro of murine (Friend) virus-induced leukemic cells.小鼠（弗瑞德）病毒诱导的白血病细胞在体外的红细胞成熟过程。

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Biochemistry. 1971 Aug 3;10(16):3014-21. doi: 10.1021/bi00792a005.

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On the lability of poly(A) sequences during extraction of messenger RNA from polyribosomes.关于从多核糖体中提取信使核糖核酸过程中多聚腺苷酸序列的不稳定性。

Biochim Biophys Acta. 1972 Mar 14;262(2):220-6. doi: 10.1016/0005-2787(72)90236-5.

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Eur J Biochem. 1973 Feb 15;33(1):192-200. doi: 10.1111/j.1432-1033.1973.tb02669.x.

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Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.通过寡聚胸苷酸纤维素柱层析法纯化具有生物活性的珠蛋白信使核糖核酸。

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