Kim K, Bloom M S, Browne R W, Bell E M, Yucel R M, Fujimoto V Y
Department of Environmental Health Sciences, University at Albany, State University of New York, Rensselaer, NY, USA.
Department of Epidemiology and Biostatistics, University at Albany, State University of New York, Rensselaer, NY, USA.
J Assist Reprod Genet. 2017 Jan;34(1):1-10. doi: 10.1007/s10815-016-0826-x. Epub 2016 Nov 29.
Follicular redox balance is likely to be important for embryo quality during in vitro fertilization (IVF), and the anti-oxidative high desity lipoprotein (HDL) particle is the sole lipoprotein measured in follicular fluid (FF). Therefore, we investigated FF HDL particle components as predictors of embryo quality during IVF.
Two research follicles collected from each participant were individually tracked, and 103 women having at least one developed embryo were included in the analysis. Concentrations of 15 non-cholesterol HDL particle components and 26 HDL-cholesterol (HDL-C) particle size subfractions were determined. Embryo quality was assessed for embryo cell number, embryo fragmentation, and embryo symmetry. Multivariable Poisson regression with a sandwich variance estimator was used to evaluate associations between HDL particle components and embryo quality, adjusted for covariates.
Higher γ-tocopherol concentration was associated with less embryo fragmentation (relative risk [RR] = 4.43; 95 % confidence interval [CI] 1.78, 11.06), and higher apolipoprotein A-1 concentration was associated with full embryo symmetry (RR = 3.92; 95 % CI 1.56, 9.90). Higher concentrations of HDL-C subfractions in the large and medium particle size ranges were associated with poorer embryo quality.
FF HDL lipophilic micronutrients and protein components, as well as HDL-C particle size, may be important predictors of embryo quality during IVF.
卵泡氧化还原平衡可能对体外受精（IVF）期间的胚胎质量很重要，而抗氧化高密度脂蛋白（HDL）颗粒是卵泡液（FF）中唯一可测量的脂蛋白。因此，我们研究了FF HDL颗粒成分作为IVF期间胚胎质量的预测指标。
对每位参与者收集的两个研究卵泡进行单独跟踪，分析纳入了103名至少有一个发育胚胎的女性。测定了15种非胆固醇HDL颗粒成分的浓度和26种HDL胆固醇（HDL-C）颗粒大小亚组分。根据胚胎细胞数量、胚胎碎片和胚胎对称性评估胚胎质量。使用带有三明治方差估计器的多变量泊松回归来评估HDL颗粒成分与胚胎质量之间的关联，并对协变量进行调整。
较高的γ-生育酚浓度与较少的胚胎碎片相关（相对风险[RR]=4.43；95%置信区间[CI]1.78，11.06），较高的载脂蛋白A-1浓度与完全胚胎对称相关（RR=3.92；95%CI 1.56，9.90）。大、中粒径范围内较高的HDL-C亚组分浓度与较差的胚胎质量相关。
FF HDL亲脂性微量营养素和蛋白质成分以及HDL-C颗粒大小可能是IVF期间胚胎质量的重要预测指标。
