Rethinam Senthil, Nivedita Prabakaran, Hemalatha Thiagarajan, Weslen Vedakumari Sathyaraj, Sastry Thotapalli Parvathaleswara
Biological Materials Lab, CSIR-Central Leather Research Institute, Chennai, Tamilnadu - India.
Department of Biotechnology, SRM University, Kattankulathur, Chennai, Tamilnadu - India.
Int J Artif Organs. 2016 Nov 29;39(10):509-517. doi: 10.5301/ijao.5000531. Epub 2016 Nov 28.
Bluefin Trevally (Caranx melampygus) fish is mainly used for fillet production, the bones of which are discarded as a major solid waste in the fish food processing industry. In the present study, novel collagen films were prepared using the bones of Bluefin Trevally (BT). The study investigates the potential of using this collagen film as a wound dressing material.
The prepared collagen films (CFs) were characterized for their physicochemical properties using Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), scanning electron microscopy (SEM), atomic force microscopy (AFM), tensile strength, elongation at break, etc. In vitro studies using human keratinocyte cell line (HaCaT) also proved the biocompatibility of CF. The CFs were used as wound dressing material on the experimental wounds of rats and the healing pattern was evaluated using planimetric and histopathological studies.
CF prepared from the bones of BT possessed better mechanical properties. The in vitro studies demonstrated its biocompatible nature. Acceleration of wound healing in CF-treated rats was evident in the in vivo studies.
The study has devised a process for using fish waste in the preparation of a value-added product like wound dressing material. The CF with the required strength, biocompatibility and wound healing properties may be tried as a wound dressing material in large animals after obtaining the necessary approval.
蓝鳍鲹（Caranx melampygus）主要用于鱼片生产，其鱼骨在鱼类食品加工业中作为主要固体废物被丢弃。在本研究中，使用蓝鳍鲹（BT）的鱼骨制备了新型胶原膜。该研究调查了使用这种胶原膜作为伤口敷料材料的潜力。
使用傅里叶变换红外光谱（FTIR）、热重分析（TGA）、扫描电子显微镜（SEM）、原子力显微镜（AFM）、拉伸强度、断裂伸长率等对制备的胶原膜（CFs）的物理化学性质进行了表征。使用人角质形成细胞系（HaCaT）的体外研究也证明了CF的生物相容性。将CFs用作大鼠实验伤口的敷料材料，并使用平面测量和组织病理学研究评估愈合模式。
由BT鱼骨制备的CF具有更好的机械性能。体外研究证明了其生物相容性。体内研究表明，CF治疗的大鼠伤口愈合加速。
该研究设计了一种利用鱼类废弃物制备如伤口敷料材料等增值产品的工艺。在获得必要批准后，具有所需强度、生物相容性和伤口愈合特性的CF可尝试用作大型动物的伤口敷料材料。
