Senthil Rethinam, Berly Robert, Bhargavi Ram Thimmiah, Gobi Nallathambi
1 Department of Textile Technology, Anna University, Chennai, India.
2 Biological Material Laboratory, Central Leather Research Institute, Chennai, India.
Int J Artif Organs. 2018 Aug;41(8):467-473. doi: 10.1177/0391398818775949. Epub 2018 May 30.
In this study, a blend of synthetic polymer (poly(vinyl) alcohol), natural polymer (collagen type I from fish bone), and graphene oxide nanoparticles is used to fabricate a composite nanofibrous scaffold, by electrospinning, for their potential application in accelerated wound healing.
The scaffold was characterized for its physicochemical and mechanical properties. In vitro studies were carried out using human keratinocyte cell line (HaCaT) which proved the biocompatibility of the scaffold. In vivo study using mice model was carried out and the healing pattern was evaluated using histopathological studies.
Scaffold prepared from poly(vinyl) alcohol, collagen type I from fish bone, and graphene oxide possessed better physicochemical and mechanical properties. In addition, in vivo and in vitro studies showed its accelerated wound healing properties.
The scaffold with required strength and biocompatibility may be tried as a wound dressing material in large animals after getting necessary approval.
在本研究中，通过静电纺丝法将合成聚合物（聚乙烯醇）、天然聚合物（鱼骨来源的I型胶原蛋白）和氧化石墨烯纳米颗粒混合，制备一种复合纳米纤维支架，以探索其在加速伤口愈合方面的潜在应用。
对该支架的物理化学和力学性能进行表征。使用人角质形成细胞系（HaCaT）进行体外研究，证实了该支架的生物相容性。利用小鼠模型进行体内研究，并通过组织病理学研究评估愈合模式。
由聚乙烯醇、鱼骨来源的I型胶原蛋白和氧化石墨烯制备的支架具有更好的物理化学和力学性能。此外，体内和体外研究均显示其具有加速伤口愈合的特性。
在获得必要批准后，具有所需强度和生物相容性的该支架可尝试作为大型动物的伤口敷料材料。
