Park Sooyeon, Park Ji-Min, Lee Jung-Sook, Yoon Jung-Hoon
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
Korean Collection for Type Cultires (KCTC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), 181 Ipsin-gil, Jeongeup, South Korea.
Arch Microbiol. 2019 Mar;201(2):215-222. doi: 10.1007/s00203-018-1599-7. Epub 2018 Nov 27.
A lipolytic, Gram-negative, aerobic, non-motile and pleomorphic bacterial strain, designated W-SW3, was isolated from seawater around Wando, an island of South Korea, and its taxonomic position was determined using a polyphasic approach. Its optimal growth occurred at 30 °C, pH 7.0-8.0 and 2.0-4.0% (w/v) NaCl. Neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that strain W-SW3 belonged to the genus Roseovarius of the family Rhodobacteraceae, by showing the highest 16S rRNA gene sequence similarity value (97.3%) to the type strain of Roseovarius nanhaiticus. Strain W-SW3 exhibited 16S rRNA gene sequence similarities of 93.2-95.6% to the type strains of the other Roseovarius species. Ubiquinone-10 was found as the predominant ubiquinone and Cω7c and C were found as the major fatty acids. Phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified lipid and one unidentified aminolipid were major polar lipids detected. The DNA G + C content of strain W-SW3 was 63.9 mol%. Mean DNA-DNA relatedness values of strain W-SW3 with the type strains of R. nanhaiticus and R. antarcticus were 18 and 9%, respectively. The phenotypic properties, together with the phylogenetic and genetic data, made it possible strain W-SW3 to be separated from other recognized species of the genus Roseovarius. On the basis of the data presented, strain W-SW3 represents a novel species of the genus Roseovarius, for which the name Roseovarius ponticola sp. nov. is proposed. The type strain is W-SW3 (= KCTC 62424 = KACC 19615 = NBRC 113186).
从韩国莞岛附近海域分离出一株具有脂肪分解能力、革兰氏阴性、需氧、不运动且多形态的细菌菌株，命名为W-SW3，并采用多相分类法确定其分类地位。其最适生长温度为30℃，pH值为7.0 - 8.0，NaCl浓度为2.0 - 4.0%（w/v）。基于16S rRNA基因序列构建的邻接法系统发育树表明，菌株W-SW3属于红杆菌科的玫瑰变色菌属，与南海玫瑰变色菌模式菌株的16S rRNA基因序列相似性最高（97.3%）。菌株W-SW3与其他玫瑰变色菌属模式菌株的16S rRNA基因序列相似性为93.2 - 95.6%。主要泛醌为泛醌-10，主要脂肪酸为Cω7c和C。检测到的主要极性脂为磷脂酰胆碱、磷脂酰甘油、双磷脂酰甘油、一种未鉴定的脂质和一种未鉴定的氨基脂质。菌株W-SW3的DNA G + C含量为63.9 mol%。菌株W-SW3与南海玫瑰变色菌和南极玫瑰变色菌模式菌株的平均DNA - DNA杂交值分别为18%和9%。表型特征以及系统发育和遗传数据表明，菌株W-SW3可与玫瑰变色菌属其他已确认的物种区分开来。根据所提供的数据，菌株W-SW3代表玫瑰变色菌属的一个新物种，提议将其命名为Roseovarius ponticola sp. nov.。模式菌株为W-SW3（= KCTC 62424 = KACC 19615 = NBRC 113186）。
