Park Sooyeon, Yoon Sun Young, Ha Min-Ji, Jung Yong-Taek, Yoon Jung-Hoon
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
University of Science and Technology (UST), 113 Gwahak-ro, Yuseong-gu, Daejeon, Republic of Korea.
Int J Syst Evol Microbiol. 2016 Dec;66(12):5550-5555. doi: 10.1099/ijsem.0.001555. Epub 2016 Oct 9.
A Gram-stain-negative, aerobic, non-spore-forming, motile and coccoid, ovoid or rod-shaped bacterial strain, designated OITF-36T, was isolated from a tidal flat in Oido, an island of South Korea, and subjected to a polyphasic taxonomic study. Strain OITF-36T grew optimally at 30 °C, at pH 7.0-8.0 and in the presence of 2.0-3.0 % (w/v) NaCl. Neighbour-joining and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences revealed that strain OITF-36T clustered with the type strains of Primorskyibactersedentarius and Primorskyibacter insulae, showing 97.4-97.6 % sequence similarity. It exhibited sequence similarity values of less than 96.9 % to the type strains of the other recognized species. Strain OITF-36T contained Q-10 as the predominant ubiquinone and C18 : 1ω7c as the major fatty acid. The major polar lipids detected in strain OITF-36T were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, one unidentified aminolipid and one unidentified lipid. The DNA G+C content of strain OITF-36T was 61.8 mol%, and its mean DNA-DNA relatedness values with the type strains of P. sedentarius and P. insulae were 16-24 %. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain OITF-36T is separate from P. sedentarius and P. insulae. On the basis of the data presented, strain OITF-36T is considered to represent a novel species of the genus Primorskyibacter, for which the name Primorskyibacteraestuariivivens sp. nov. is proposed. The type strain is OITF-36T (=KCTC 52455T=NBRC 112419T).
从韩国岛屿梧岛的潮滩中分离出一株革兰氏染色阴性、需氧、无芽孢形成、运动性且呈球状、卵形或杆状的细菌菌株，命名为OITF-36T，并对其进行了多相分类研究。菌株OITF-36T在30°C、pH 7.0 - 8.0以及2.0 - 3.0%（w/v）NaCl存在的条件下生长最佳。基于16S rRNA基因序列构建的邻接法和最大简约法系统发育树显示，菌株OITF-36T与定居滨海边疆杆菌（Primorskyibacter sedentarius）和岛屿滨海边疆杆菌（Primorskyibacter insulae）的模式菌株聚类在一起，序列相似性为97.4 - 97.6%。它与其他已确认物种的模式菌株的序列相似性值低于96.9%。菌株OITF-36T以Q-10作为主要泛醌，以C18 : 1ω7c作为主要脂肪酸。在菌株OITF-36T中检测到的主要极性脂类为磷脂酰胆碱、磷脂酰甘油、磷脂酰乙醇胺、磷脂酰单甲基乙醇胺、一种未鉴定的氨基脂类和一种未鉴定的脂类。菌株OITF-36T的DNA G+C含量为61.8 mol%，其与定居滨海边疆杆菌和岛屿滨海边疆杆菌模式菌株的平均DNA-DNA相关性值为16 - 24%。不同的表型特征，以及系统发育和遗传独特性，表明菌株OITF-36T与定居滨海边疆杆菌和岛屿滨海边疆杆菌不同。根据所提供的数据，菌株OITF-36T被认为代表了滨海边疆杆菌属的一个新物种，为此提议将其命名为河口滨海边疆杆菌（Primorskyibacter aestuariivivens sp. nov.）。模式菌株为OITF-36T（=KCTC 52455T=NBRC 1
