Oh Mihee, Kim Jong-Hwa, Kim Wonyong
Department of Microbiology, Chung-Ang University College of Medicine, Seoul, Republic of Korea.
Int J Syst Evol Microbiol. 2016 Dec;66(12):5514-5518. doi: 10.1099/ijsem.0.001549. Epub 2016 Oct 7.
A Gram-staining-negative, aerobic, non-motile, rod-shaped bacterial strain, designated CAU 1062T, was isolated from marine sand in Jeju island, Republic of Korea and its taxonomic position was investigated using a polyphasic approach. CAU 1062T grew optimally at 30 °C and pH 8 in the presence of 2 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that CAU 1062T formed a distinct lineage within the genus Tropicimonasand was the most closely related to Tropicimonas sediminicola M97T (similarity 96.11 %). The strain had ubiquinone-10 (Q-10) as the predominant respiratory quinone and C18 : 1ω7c as the major cellular fatty acid. The polar lipid pattern of CAU 1062T consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, an aminolipid, six phospholipids and five lipids. The DNA G+C content was 65.7 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, CAU 1062T represents a novel species of the genus Tropicimonas, for which the name Tropicimonas arenosa sp. nov. is proposed. The type strain is CAU 1062T (=KCTC 52178T=NBRC 111995T).
从韩国济州岛的海沙中分离出一株革兰氏染色阴性、需氧、无运动性、杆状的细菌菌株，命名为CAU 1062T，并采用多相分类法研究其分类地位。CAU 1062T在2%（w/v）NaCl存在的条件下，于30°C和pH 8时生长最佳。基于16S rRNA基因序列的系统发育分析表明，CAU 1062T在嗜温单胞菌属内形成了一个独特的分支，与沉积嗜温单胞菌M97T关系最为密切（相似度为96.11%）。该菌株以泛醌-10（Q-10）作为主要呼吸醌，以C18:1ω7c作为主要细胞脂肪酸。CAU 1062T的极性脂质模式由双磷脂酰甘油、磷脂酰甘油、磷脂酰胆碱、一种氨基脂质、六种磷脂和五种脂质组成。DNA G+C含量为65.7 mol%。基于表型、化学分类和系统发育数据，CAU 1062T代表嗜温单胞菌属的一个新物种，为此提出新名称沙嗜温单胞菌（Tropicimonas arenosa sp. nov.）。模式菌株为CAU 1062T（=KCTC 52178T=NBRC 111995T）。
