Thongphrom Chutimon, Kim Jong-Hwa, Yoon Jung-Hoon, Bora Nagamani, Kim Wonyong
Department of Microbiology, Chung-Ang University College of Medicine, Seoul, Republic of Korea.
Department of Food Science and Biotechnology, Sungkyunkwan University, Suwon, Republic of Korea.
Int J Syst Evol Microbiol. 2017 Jan;67(1):121-126. doi: 10.1099/ijsem.0.001581. Epub 2017 Feb 20.
A Gram-stain-negative, non-motile, non-spore-forming, aerobic and short rod-shaped bacterial strain, CAU 1311T, was isolated from sea sand in the Republic of Korea. Strain CAU 1311T grew at temperatures from 20-37 °C, in the range of pH 6.5-10.0, and under various NaCl concentrations from 0-6 % (w/v). Phylogenetic analysis based on the 16S rRNA gene sequence of CAU 1311T showed that it formed a distinct lineage within the family Rhodobacteraceae as a separate deep branch, with 96.2 % or lower sequence identity to representatives of the genera Marivita, Aestuariivita, Mameliella, Sulfitobacter and Maritimibacter. The major fatty acid was C18 : 1ω7c and the predominant respiratory quinone was Q-10. The major polar lipids were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminolipid and an unidentified glycolipid. The DNA G+C content was 60.7 mol%. On the basis of genotypic, phenotypic and chemotaxanomic findings, strain CAU 1311T could be classified as representing a novel species of a new genus of the family Rhodobacteraceae, for which the name Marimonas arenosa gen. nov., sp. nov. is proposed. The type strain of the type species is CAU 1311T (=KCTC 52189T=NBRC 111988T).
从韩国海砂中分离出一株革兰氏染色阴性、无运动性、不形成芽孢、需氧的短杆状细菌菌株CAU 1311T。菌株CAU 1311T在20-37°C的温度、pH 6.5-10.0的范围内以及0-6%（w/v）的各种NaCl浓度下均可生长。基于菌株CAU 1311T的16S rRNA基因序列的系统发育分析表明，它在红杆菌科内形成了一个独特的分支，作为一个单独的深分支，与Marivita、Aestuariivita、Mameliella、Sulfitobacter和Maritimibacter属的代表菌株的序列同一性为96.2%或更低。主要脂肪酸为C18 : 1ω7c，主要呼吸醌为Q-10。主要极性脂为磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰甘油、一种未鉴定的氨基脂和一种未鉴定的糖脂。DNA G+C含量为60.7 mol%。基于基因型、表型和化学分类学结果，菌株CAU 1311T可被归类为代表红杆菌科一个新属的一个新物种，为此提出新属名Marimonas，新种名arenosa。新物种的模式菌株为CAU 1311T（=KCTC 52189T=NBRC 111988T）。
