Department of Microbiology, Chung-Ang University College of Medicine, Seoul 06974, Republic of Korea.
Department of Food Science and Biotechnology, Sungkyunkwan University, Suwon, Republic of Korea.
Int J Syst Evol Microbiol. 2020 Dec;70(12):6323-6330. doi: 10.1099/ijsem.0.004534. Epub 2020 Oct 29.
A Gram-stain-negative, non-pigmented, non-spore-forming, motile, strictly aerobic bacterial strain, designated CAU 1492, was isolated from a coastal sand dune and its taxonomic position was examined using a polyphasic approach. Cells of strain CAU 1492 grew optimally at 30 °C, pH 7.0 and in 3 % (w/v) NaCl. Phylogenetic analysis based on the 16S rRNA gene sequence of CAU 1492 showed that it formed a distinct lineage within the family as a separate deep branch, with 96.8 % or lower sequence similarity values to representatives of the genera , , , , , , and . Strain CAU 1492 was closely related to DPG-138 (96.8 %), SW-277 (96.7 %), SCM-1 (96.7 %), FF3 (96.6 %) and BB-MW15 (96.4 %) based on 16S rRNA gene sequences. The major cellular fatty acids of strain CAU 1492 were cyclo-C 8 and summed feature 8 (C 7/C 6). The polar lipid pattern was composed of phosphatidylglycerol, phosphatidylethanolamine, an unidentified phospholipid and an unidentified aminolipid. The strain contained Q-10 as the sole respiratory quinone. The draft genome of strain CAU 1492 was 4.63 Mb with a DNA G+C content of 63.1 mol%. The genome includes 4292 protein-coding genes and a five rRNA operons. On the basis of the phenotypic, chemotaxonomic and genomic data, strain CAU 1492 represents a novel genus in the family for which the name gen. nov., sp. nov. is proposed. The type strain of is CAU 1492 (=KCTC 62998=NBRC 113696).
一株革兰氏阴性、无色素、非孢子形成、能动、严格需氧的细菌菌株，被命名为 CAU 1492，从沿海沙丘中分离得到，采用多相分类法研究其分类地位。菌株 CAU 1492 的细胞在 30°C、pH7.0 和 3%（w/v）NaCl 中生长最佳。基于 CAU 1492 的 16S rRNA 基因序列的系统发育分析表明，它在 科内形成一个单独的深分支，与属 、 、 、 、 、 、 和 的代表菌株的序列相似性值均为 96.8%或更低。菌株 CAU 1492 与 DPG-138（96.8%）、SW-277（96.7%）、SCM-1（96.7%）、FF3（96.6%）和 BB-MW15（96.4%）基于 16S rRNA 基因序列最为密切相关。CAU 1492 菌株的主要细胞脂肪酸为环-C8 和总和特征 8（C7/C6）。极性脂谱由磷脂酰甘油、磷脂酰乙醇胺、一种未鉴定的磷脂和一种未鉴定的胺脂组成。该菌株含有 Q-10 作为唯一的呼吸醌。CAU 1492 菌株的基因组草图为 4.63Mb，GC 含量为 63.1mol%。基因组包含 4292 个蛋白编码基因和五个 rRNA 操纵子。基于表型、化学分类和基因组数据，菌株 CAU 1492 代表 科中的一个新属，建议采用新属名 ，新种名 。的模式菌株为 CAU 1492（=KCTC 62998=NBRC 113696）。
