Nedashkovskaya Olga, Otstavnykh Nadezhda, Balabanova Larissa, Bystritskaya Evgenia, Kim Song-Gun, Zhukova Natalia, Tekutyeva Liudmila, Isaeva Marina
G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Prospect 100 Let Vladivostoku, 159, Vladivostok 690022, Russia.
Korean Collection for Type Cultures, Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, 181 Ipsin-gil, Jeongeup-si 56212, Republic of Korea.
Microorganisms. 2023 Sep 30;11(10):2463. doi: 10.3390/microorganisms11102463.
A novel Gram-staining negative, strictly aerobic, rod-shaped, and non-motile bacterium, designated strain 10Alg 79, was isolated from the red alga . A phylogenetic analysis based on 16S rRNA gene sequences placed the novel strain within the family , class , phylum , where the nearest neighbor was ZQ172 (97.33% of identity). However, a phylogenomic study clearly showed that strain 10Alg 79 forms a distinct evolutionary lineage at the genus level within the family combining with strains L1 8-17, KCTC 52189, and DSM 24677. The ANI, AAI, and dDDH values between them were 75.63-78.15%, 67.41-73.08%, and 18.8-19.8%, respectively. The genome comprises 3,754,741 bp with a DNA GC content of 62.1 mol%. The prevalent fatty acids of strain 10Alg 79 were C and C. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, an unidentified aminolipid, an unidentified phospholipid and an unidentified lipid. A pan-genome analysis showed that the unique part of the 10Alg 79 genome consists of 13 genus-specific clusters and 413 singletons. The annotated singletons were more often related to transport protein systems, transcriptional regulators, and enzymes. A functional annotation of the draft genome sequence revealed that this bacterium could be a source of a new phosphorylase, which may be used for phosphoglycoside synthesis. A combination of the genotypic and phenotypic data showed that the bacterial isolate represents a novel species and a novel genus, for which the name gen. nov., sp. nov. is proposed. The type strain is 10Alg 79 (=KCTC 72611 = KMM 6723).
从红藻中分离出一株新型革兰氏染色阴性、严格需氧、杆状且无运动性的细菌，命名为菌株10Alg 79。基于16S rRNA基因序列的系统发育分析将该新菌株置于 科、 纲、 门内，其最接近的亲缘种是ZQ172（同一性为97.33%）。然而，一项全基因组系统发育研究清楚地表明，菌株10Alg 79与菌株L1 8 - 17、KCTC 52189和DSM 24677在 科内的属水平上形成了一个独特的进化谱系。它们之间的ANI、AAI和dDDH值分别为75.63 - 78.15%、67.41 - 73.08%和18.8 - 19.8%。该基因组由3,754,741 bp组成，DNA的GC含量为62.1 mol%。菌株10Alg 79的主要脂肪酸为C和C。极性脂质谱由磷脂酰乙醇胺、磷脂酰甘油、磷脂酰胆碱、一种未鉴定的氨基脂质、一种未鉴定的磷脂和一种未鉴定的脂质组成。泛基因组分析表明，10Alg 79基因组的独特部分由13个属特异性簇和413个单拷贝基因组成。注释的单拷贝基因更多地与转运蛋白系统、转录调节因子和酶相关。对基因组草图序列的功能注释表明，这种细菌可能是一种新的磷酸化酶的来源，可用于磷酸糖苷合成。基因型和表型数据的结合表明，该分离细菌代表一个新物种和一个新属，为此提出名称 属，新属， 种，新种。模式菌株为10Alg 79（=KCTC 72611 = KMM 6723）。
