Department of Microbiology, Faculty of Science, Kasetsart University, Chatuchak, Bangkok 10900, Thailand.
Biodiversity Center, Kasetsart University (BDCKU), Chatuchak, Bangkok 10900, Thailand.
Int J Syst Evol Microbiol. 2021 Jul;71(7). doi: 10.1099/ijsem.0.004849.
A novel actinomycete, designated strain KK5PA1, was isolated from a soil sample collected from Kuan Kreng peat swamp forest, Nakhon Si Thammarat Province, Thailand. The morphological, chemotaxonomic and phylogenetic characteristics were consistent with its classification in the genus . Strain KK5PA1 was most closely related to NEAU-HZ10 (98.0 %) and 701 (97.6 %). The G+C content of the genomic DNA was 72.3 mol%. Digital DNA-DNA hybridization and average nucleotide identity values between the genome sequence of strain KK5PA1 and those of DSM 42138(25.1 and 79.1 %) and DSM 41944(25.1 and 79.7%) were below the thresholds of 70 and 96 % for prokaryotic conspecific assignation. Chemotaxonomic data revealed that strain KK5PA1 possessed MK-9(H) and MK-9(H) as the predominant menaquinones. It contained lldiaminopimelic acid as the diagnostic diamino acid and galactose, glucose, mannose and ribose as whole-cell sugars. The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol mannoside, two unidentified aminolipids, five unidentified phospholipids and an unidentified lipid. The predominant cellular fatty acids were iso-C, anteiso-C and C. On the basis of these genotypic and phenotypic data, it is proposed that strain KK5PA1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is strain KK5PA1 (=TBRC 13094=NBRC 114802).
一株新的放线菌，命名为 KK5PA1 菌株，从泰国那空是贪玛叻府宽克拉彭泥炭沼泽森林的土壤样本中分离得到。形态学、化学分类学和系统发育特征均符合其在属内的分类。KK5PA1 菌株与 NEAU-HZ10（98.0%）和 701（97.6%）最为密切相关。基因组 DNA 的 G+C 含量为 72.3mol%。KK5PA1 菌株与 DSM 42138（25.1%和 79.1%）和 DSM 41944（25.1%和 79.7%）基因组序列的数字 DNA-DNA 杂交值和平均核苷酸同一性值均低于 70%和 96%，这是原核生物同种鉴定的阈值。化学分类学数据表明，KK5PA1 菌株含有 MK-9(H)和 MK-9(H)作为主要的menaquinone。它含有 lldiaminopimelic 酸作为特征性二氨基庚二酸，全细胞糖含有半乳糖、葡萄糖、甘露糖和核糖。极性脂由双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰肌醇甘露糖苷、两种未鉴定的氨基脂、五种未鉴定的磷脂和一种未鉴定的脂组成。主要的细胞脂肪酸为 iso-C、anteiso-C 和 C。基于这些基因型和表型数据，建议 KK5PA1 菌株代表一个新的属种，命名为 sp. nov.。其模式菌株为 KK5PA1 菌株（=TBRC 13094=NBRC 114802）。
