Wang Ting-Gang, Xu Jie, Zhu Ai-Hua, Lu Hua, Miao Zong-Ning, Zhao Peng, Hui Guo-Zhen, Wu Wei-Jiang
Department of Neurology, Wuxi Third People's Hospital, Wuxi, Jiangsu Province, China.
Department of Neurosurgery, Wuxi Third People's Hospital, Wuxi, Jiangsu Province, China.
Neural Regen Res. 2016 Oct;11(10):1670-1677. doi: 10.4103/1673-5374.193249.
Treatment and functional reconstruction after central nervous system injury is a major medical and social challenge. An increasing number of researchers are attempting to use neural stem cells combined with artificial scaffold materials, such as fibroin, for nerve repair. However, such approaches are challenged by ethical and practical issues. Amniotic tissue, a clinical waste product, is abundant, and amniotic epithelial cells are pluripotent, have low immunogenicity, and are not the subject of ethical debate. We hypothesized that amniotic epithelial cells combined with silk fibroin scaffolds would be conducive to the repair of spinal cord injury. To test this, we isolated and cultured amniotic epithelial cells, and constructed complexes of these cells and silk fibroin scaffolds. Implantation of the cell-scaffold complex into a rat model of spinal cord injury resulted in a smaller glial scar in the damaged cord tissue than in model rats that received a blank scaffold, or amniotic epithelial cells alone. In addition to a milder local immunological reaction, the rats showed less inflammatory cell infiltration at the transplant site, milder host-versus-graft reaction, and a marked improvement in motor function. These findings confirm that the transplantation of amniotic epithelial cells combined with silk fibroin scaffold can promote the repair of spinal cord injury. Silk fibroin scaffold can provide a good nerve regeneration microenvironment for amniotic epithelial cells.
中枢神经系统损伤后的治疗与功能重建是一项重大的医学和社会挑战。越来越多的研究人员试图将神经干细胞与诸如丝素蛋白等人工支架材料相结合来进行神经修复。然而，此类方法面临着伦理和实际问题的挑战。羊膜组织作为一种临床废弃物，来源丰富，且羊膜上皮细胞具有多能性、免疫原性低，并非伦理争议的对象。我们推测羊膜上皮细胞与丝素蛋白支架相结合将有助于脊髓损伤的修复。为验证这一点，我们分离并培养了羊膜上皮细胞，并构建了这些细胞与丝素蛋白支架的复合物。将细胞 - 支架复合物植入大鼠脊髓损伤模型后，受损脊髓组织中的胶质瘢痕比接受空白支架或单独植入羊膜上皮细胞的模型大鼠更小。除了局部免疫反应较轻外，大鼠在移植部位的炎性细胞浸润较少，宿主 - 移植物反应较轻，运动功能有显著改善。这些发现证实，羊膜上皮细胞与丝素蛋白支架联合移植可促进脊髓损伤的修复。丝素蛋白支架可为羊膜上皮细胞提供良好的神经再生微环境。
