Gansu University of Chinese Medicine, College of Integrated Traditional Chinese and Western Medicine - Gansu Province, China.
Pingjin Hospital Brain Center, Tianjin Key Laboratory of Neurotrauma Repair, Characteristic Medical Center of Chinese People's Armed Police Force - Tianjin, China.
Rev Assoc Med Bras (1992). 2021 Sep;67(9):1342-1348. doi: 10.1590/1806-9282.20200697.
This study aimed to assess the effect of the collagen/silk fibroin scaffolds seeded with human umbilical cord-mesenchymal stem cells on functional recovery after acute complete spinal cord injury.
The fibroin and collagen were mixed (mass ratio, 3:7), and the composite scaffolds were produced. Forty rats were randomly divided into the Sham group (without spinal cord injury), spinal cord injury group (spinal cord transection without any implantation), collagen/silk fibroin scaffolds group (spinal cord transection with implantation of the collagen/silk fibroin scaffolds), and collagen/silk fibroin scaffolds + human umbilical cord-mesenchymal stem cells group (spinal cord transection with the implantation of the collagen/silk fibroin scaffolds co-cultured with human umbilical cord-mesenchymal stem cells). Motor evoked potential, Basso-Beattie-Bresnahan scale, modified Bielschowsky's silver staining, and immunofluorescence staining were performed.
The BBB scores in the collagen/silk fibroin scaffolds + human umbilical cord-mesenchymal stem cells group were significantly higher than those in the spinal cord injury and collagen/silk fibroin scaffolds groups (p<0.05 or p<0.01). The amplitude and latency were markedly improved in the collagen/silk fibroin scaffolds + human umbilical cord-mesenchymal stem cells group compared with the spinal cord injury and collagen/silk fibroin scaffolds groups (p<0.05 or p<0.01). Meanwhile, compared to the spinal cord injury and collagen/silk fibroin scaffolds groups, more neurofilament positive nerve fiber ensheathed by myelin basic protein positive structure at the injury site were observed in the collagen/silk fibroin scaffolds + human umbilical cord-mesenchymal stem cells group (p<0.01, p<0.05). The results of Bielschowsky's silver staining indicated more nerve fibers was observed at the lesion site in the collagen/silk fibroin scaffolds + human umbilical cord-mesenchymal stem cells group compared with the spinal cord injury and collagen/silk fibroin scaffolds groups (p<0.01, p< 0.05).
The results demonstrated that the transplantation of human umbilical cord-mesenchymal stem cells on a collagen/silk fibroin scaffolds could promote nerve regeneration, and recovery of neurological function after acute spinal cord injury.
本研究旨在评估人脐带间充质干细胞种植于胶原/丝素纤维支架后对急性完全性脊髓损伤后功能恢复的影响。
将丝素和胶原按质量比 3：7 混合，制备复合支架。将 40 只大鼠随机分为假手术组（无脊髓损伤）、脊髓损伤组（脊髓横断无任何植入物）、胶原/丝素纤维支架组（脊髓横断植入胶原/丝素纤维支架）和胶原/丝素纤维支架+人脐带间充质干细胞组（脊髓横断植入与人脐带间充质干细胞共培养的胶原/丝素纤维支架）。进行运动诱发电位、Basso-Beattie-Bresnahan 量表、改良 Bielschowsky 银染和免疫荧光染色。
胶原/丝素纤维支架+人脐带间充质干细胞组 BBB 评分明显高于脊髓损伤组和胶原/丝素纤维支架组（p<0.05 或 p<0.01）。胶原/丝素纤维支架+人脐带间充质干细胞组的振幅和潜伏期明显优于脊髓损伤组和胶原/丝素纤维支架组（p<0.05 或 p<0.01）。同时，与脊髓损伤组和胶原/丝素纤维支架组相比，胶原/丝素纤维支架+人脐带间充质干细胞组损伤部位可见更多被髓鞘碱性蛋白阳性结构包裹的神经丝阳性神经纤维（p<0.01，p<0.05）。Bielschowsky 银染结果表明，胶原/丝素纤维支架+人脐带间充质干细胞组损伤部位的神经纤维较脊髓损伤组和胶原/丝素纤维支架组更多（p<0.01，p<0.05）。
研究结果表明，人脐带间充质干细胞在胶原/丝素纤维支架上的移植可促进急性脊髓损伤后神经再生和神经功能恢复。
