生物支架调控对软骨细胞增殖及关节软骨修复的影响
Influence of biological scaffold regulation on the proliferation of chondrocytes and the repair of articular cartilage.
作者信息
Wang Si-Qun, Xia Jun, Chen Jie, Lu Jian-Xi, Wei Yi-Bing, Chen Fei-Yan, Huang Gang-Yong, Shi Jing-Sheng, Yu Yong-Lin
机构信息
Department of Orthopaedics, Huashan Hospital of Fudan University Shanghai 200040, China.
Shanghai Bio-Lu Biomaterials Co. Ltd Shanghai 201114, China.
出版信息
Am J Transl Res. 2016 Nov 15;8(11):4564-4573. eCollection 2016.
PURPOSE
To investigate the effects of hard tissue engineering scaffold (the material is β-TCP) with different micro-structures on the proliferation of chondrocytes, and the influence of its composite erythrocytes on the repair of articular cartilage defects.
METHODS
Rabbit cartilage cells were on β-TCP bioceramic scaffold with different micro-structures in vitro, the proliferation growth trend of chondrocytes within the scaffold was calculated, and a optimal micro-structure suitable for cartilage cell growth was determined. Composite chondrocytes were implanted into rabbit models of articular cartilage defects, and the repair situation was observed.
RESULTS
the bioceramic scaffold with an inner diameter of 120 μm and an aperture of 500-630 μm was suitable for the growth of cartilage cells. Scaffold loaded with second generation of cartilage cell suspension got a top histological score of 20.76±2.13, which was closely similar to that of normal cartilage.
CONCLUSION
When loaded with the second generation of cartilage cells, the β-TCP biological ceramic scaffold with a pore size of 500-630 μm, and an inner diameter of 120 μm, shows a best repairing effect on animal articular cartilage defects.
目的
研究不同微观结构的硬组织工程支架（材料为β - 磷酸三钙）对软骨细胞增殖的影响，以及其复合红细胞对关节软骨缺损修复的影响。
方法
将兔软骨细胞置于具有不同微观结构的β - 磷酸三钙生物陶瓷支架上进行体外培养，计算支架内软骨细胞的增殖生长趋势，确定适合软骨细胞生长的最佳微观结构。将复合软骨细胞植入兔关节软骨缺损模型中，观察修复情况。
结果
内径为120μm、孔径为500 - 630μm的生物陶瓷支架适合软骨细胞生长。负载第二代软骨细胞悬液的支架组织学评分最高，为20.76±2.13，与正常软骨相近。
结论
负载第二代软骨细胞时，孔径为500 - 630μm、内径为120μm的β - 磷酸三钙生物陶瓷支架对动物关节软骨缺损显示出最佳修复效果。
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