Wedding J L, Harris H H, Bader C A, Plush S E, Mak R, Massi M, Brooks D A, Lai B, Vogt S, Werrett M V, Simpson P V, Skelton B W, Stagni S
Department of Chemistry, The University of Adelaide, SA 5005, Australia.
School of Pharmacy and Medical Sciences, University of South Australia, Adelaide, South Australia 5005, Australia.
Metallomics. 2017 Apr 19;9(4):382-390. doi: 10.1039/c6mt00243a.
Optical epifluorescence microscopy was used in conjunction with X-ray fluorescence imaging to monitor the stability and intracellular distribution of the luminescent rhenium(i) complex fac-[Re(CO)(phen)L], where phen = 1,10-phenathroline and L = 5-(4-iodophenyl)tetrazolato, in 22Rv1 cells. The rhenium complex showed no signs of ancillary ligand dissociation, a conclusion based on data obtained via X-ray fluorescence imaging aligning iodine and rhenium distributions. A diffuse reticular localisation was detected for the complex in the nuclear/perinuclear region of cells, by either optical or X-ray fluorescence imaging techniques. X-ray fluorescence also showed that the rhenium complex disrupted the homeostasis of some biologically relevant elements, such as chlorine, potassium and zinc.
光学落射荧光显微镜与X射线荧光成像结合使用，以监测发光铼（I）配合物fac-[Re(CO)(phen)L]（其中phen = 1,10-菲咯啉，L = 5-(4-碘苯基)四唑）在22Rv1细胞中的稳定性和细胞内分布。基于通过X射线荧光成像获得的碘和铼分布数据，铼配合物没有显示出辅助配体解离的迹象。通过光学或X射线荧光成像技术检测到该配合物在细胞核/核周区域呈弥漫性网状定位。X射线荧光还表明，铼配合物破坏了一些生物相关元素（如氯、钾和锌）的内稳态。
