Sun Yajing, Rajput Imran Rashid, Arain Muhammad Asif, Li Yanfei, Baloch Dost Muhammad
College of Sciences, Shantou University, China.
College of Animal Sciences and Technology, North West Agriculture and Forestry University, China.
Anim Sci J. 2017 Aug;88(8):1204-1211. doi: 10.1111/asj.12757. Epub 2016 Dec 7.
The present study evaluated the effects of Saccharomyces boulardii on duodenal digestive enzymes, morphology and cytokine induction response in broiler chicken. A total of 200 birds were allotted into two groups (n = 100) and each group divided into five replications (n = 20). The control group was fed basal diet in addition to antibiotic (virginiamycin 20 mg/kg), and treatment group received (1 × 10 colony-forming units/kg feed) S. boulardii in addition to basal diet lasting for 72 days. The results compared to control group revealed that adenosine triphosphatase, gamma glutamyl transpeptidase, lipase and trypsin activities were higher, while, no significant improvement was observed in amylase activities in the duodenum of the treatment group. Moreover, morphological findings showed that villus height, width and number of goblet cells markedly increased. Additionally, transmission electron microscopy visualized that villus height, width and structural condensation significantly increased in the treatment group. The immunohistological observations showed increased numbers of immunoglobulin A (IgA)-positive cells in the duodenum of the treatment group. Meanwhile, cytokine production levels of tumor necrosis factor-α, interleukin (IL)-10, transforming growth factor-β and secretory IgA markedly increased, and IL-6 statistically remained unchanged as compared to the control group. These findings illustrated that initial contact of S. boulardii to the duodenum has significant impact in improving enzymatic activity, intestinal morphology and cytokine response in broiler chicken.
本研究评估了布拉氏酵母菌对肉鸡十二指肠消化酶、形态及细胞因子诱导反应的影响。总共200只鸡被分为两组（n = 100），每组再分为五个重复组（n = 20）。对照组除基础日粮外还添加抗生素（维吉尼亚霉素20毫克/千克），治疗组除基础日粮外还添加（1×10⁹菌落形成单位/千克饲料）布拉氏酵母菌，持续72天。与对照组相比，结果显示治疗组十二指肠中三磷酸腺苷酶、γ-谷氨酰转肽酶、脂肪酶和胰蛋白酶活性更高，而淀粉酶活性未观察到显著改善。此外，形态学结果表明绒毛高度、宽度和杯状细胞数量显著增加。另外，透射电子显微镜观察显示治疗组绒毛高度、宽度和结构凝聚显著增加。免疫组织学观察显示治疗组十二指肠中免疫球蛋白A（IgA）阳性细胞数量增加。同时，与对照组相比，肿瘤坏死因子-α、白细胞介素（IL）-10、转化生长因子-β和分泌型IgA的细胞因子产生水平显著增加，而IL-6在统计学上保持不变。这些发现表明布拉氏酵母菌与十二指肠的初次接触对改善肉鸡的酶活性、肠道形态和细胞因子反应具有显著影响。
