Forget Aurelien, Arya Neha, Randriantsilefisoa Rotsiniaina, Miessmer Florian, Buck Marion, Ahmadi Vincent, Jonas Daniel, Blencowe Anton, Shastri V Prasad
Future Industries Institute, University of South Australia , Mawson Lakes 5095, South Australia, Australia.
Institute for Macromolecular Chemistry, University of Freiburg , 79104, Freiburg, Germany.
Biomacromolecules. 2016 Dec 12;17(12):4021-4026. doi: 10.1021/acs.biomac.6b01401. Epub 2016 Nov 22.
Hydrogel forming polysaccharides, such as the seaweed derived agarose, are well suited for wound dressing applications as they have excellent cell and soft tissue compatibility. For wound dressings, fibrous structure is desirable as the high surface area can favor adsorption of wound exudate and promote drug delivery. Although electrospinning offers a straightforward means to produce nonwoven fibrous polymeric structures, processing agarose and its derivatives into fibers through electrospinning is challenging as it has limited solubility in solvents other than water. In this study we describe the processing of carboxylated agarose (CA) fibers with antibacterial properties by electrospinning from a solution of the ionic liquid (IL) 1-butyl-3-methylimidazolium chloride ([Bmim]Cl) possessing antimicrobial properties. The extent of carboxylation was found to impact fiber diameter, mesh elastic modulus, fiber swelling, and the loading and release of IL. IL-bearing CA fibers inhibited the growth of Staphylococcus aureus and Pseudomonas aeruginosa, bacteria commonly found in wound exudate. In sum, nonwoven CA fibers processed from IL are promising as biomaterials for wound dressing applications.
形成水凝胶的多糖，如从海藻中提取的琼脂糖，因其具有出色的细胞和软组织相容性，非常适合用于伤口敷料应用。对于伤口敷料来说，纤维结构是理想的，因为高表面积有利于伤口渗出液的吸附并促进药物递送。尽管静电纺丝提供了一种生产非织造纤维聚合物结构的直接方法，但将琼脂糖及其衍生物通过静电纺丝加工成纤维具有挑战性，因为它在水以外的溶剂中的溶解度有限。在本研究中，我们描述了通过从具有抗菌性能的离子液体（IL）1-丁基-3-甲基咪唑氯盐（[Bmim]Cl）溶液中进行静电纺丝来制备具有抗菌性能的羧化琼脂糖（CA）纤维。发现羧化程度会影响纤维直径、网状弹性模量、纤维溶胀以及IL的负载和释放。含IL的CA纤维抑制了金黄色葡萄球菌和铜绿假单胞菌的生长，这两种细菌常见于伤口渗出液中。总之，由IL加工而成的非织造CA纤维作为用于伤口敷料应用的生物材料很有前景。
