Laboratory of Biomedical Vibrational Spectroscopy, University of Vale do Paraíba, 12224-000 São José dos Campos, SP, Brazil.
Laboratory of Biomedical Vibrational Spectroscopy, University of Vale do Paraíba, 12224-000 São José dos Campos, SP, Brazil.
Spectrochim Acta A Mol Biomol Spectrosc. 2017 Mar 5;174:279-285. doi: 10.1016/j.saa.2016.11.042. Epub 2016 Dec 5.
The purpose of this study is to elucidate the behavior of retinyl acetate in penetrating human skin without the presence of enhancers by using confocal Raman spectroscopy and molecular dynamics simulation.
In this study, in vivo confocal Raman spectroscopy was combined with molecular dynamics simulation to investigate the transdermal permeation of the aqueous suspension of retinyl acetate.
Permeation was measured after 30min, and retinyl acetate was found up to 20μm deep inside the stratum corneum. The delivery of retinyl acetate inside a skin membrane model was studied by molecular dynamics. The membrane model that was used represented normal young skin containing a lipid bilayer with 25% ceramide, 36% fatty acid, 30% cholesterol, and 6% cholesterol sulfate.
Spectroscopy data indicate that retinyl acetate permeates into the stratum corneum. Molecular dynamics data showed that retinyl acetate permeates in the membrane model and that their final location is deep inside the lipid bilayer. We showed, for the first time, a correlation between Raman permeation data and computational data.
本研究旨在通过共聚焦拉曼光谱和分子动力学模拟，阐明无增强剂存在时视黄醇乙酸酯在穿透人体皮肤时的行为。
本研究将体内共聚焦拉曼光谱与分子动力学模拟相结合，研究视黄醇乙酸酯水混悬液的透皮渗透。
渗透在 30 分钟后进行测量，发现视黄醇乙酸酯在角质层深处可达 20μm。通过分子动力学研究了视黄醇乙酸酯在皮肤膜模型中的输送。所使用的膜模型代表含有双层脂质的正常年轻皮肤，其中含有 25%的神经酰胺、36%的脂肪酸、30%的胆固醇和 6%的胆固醇硫酸盐。
光谱数据表明视黄醇乙酸酯渗透到角质层中。分子动力学数据显示视黄醇乙酸酯在膜模型中渗透，其最终位置在脂质双层的深处。我们首次展示了拉曼渗透数据和计算数据之间的相关性。
