Fuchsberger N, Borecký L
Acta Virol. 1978 May;22(3):238-40.
The electroimmunoassay (quantitative "rocket" immunoelectrophoresis) method was adopted for analysis and characterization of interferon preparations. Ammonium sulphate-precipitated anti-interferon globulin also containing unknown antibodies against antigens that contaminate interferon preparations was used in the tests. By comparing the results of the electroimmunoassay of fractions obtained by polyacrylamide gel electrophoresis of human leukocyte interferon with the antiviral activity of the fractions, an excellent dissociation of molecules with interferon activity from the bulk of contaminating antigens was achieved. The method is extremely sensitive and requires very small volumes for assay.
采用电免疫测定法（定量“火箭”免疫电泳）对干扰素制剂进行分析和鉴定。试验中使用了硫酸铵沉淀的抗干扰素球蛋白，其中还含有针对污染干扰素制剂的抗原的未知抗体。通过将人白细胞干扰素聚丙烯酰胺凝胶电泳所得组分的电免疫测定结果与这些组分的抗病毒活性进行比较，实现了具有干扰素活性的分子与大量污染抗原的良好分离。该方法极其灵敏，测定所需体积非常小。
