Kongkiatpaiboon Sumet, Tungsukruthai Parunkul, Sriyakool Kusuma, Pansuksan Kanoktip, Tunsirikongkon Amolnat, Pandith Hataichanok
Drug Discovery and Development Center, Thammasat University (Rangsit Campus), 99 Moo 18 Phaholyothin Road, Khlong Nueng, Khlong Luang, Pathumthani 12120, Thailand.
Chulabhorn International College of Medicine, Thammasat University, Pathumthani 12120, Thailand.
J Chromatogr Sci. 2017 Mar 1;55(3):346-350. doi: 10.1093/chromsci/bmw191.
The HPLC-DAD method was developed to determine morin content in Maclura cochinchinensis Corner heartwood extract. The chromatographic separation was performed using a Hypersil BDS C18 column, isocratic solvent system of 0.5% acetic acid in water:acetonitrile (80:20) with 1.0 mL/min flow rate and detected at 355 nm. The standard curve of morin was linear in the range of 7-905 μg/mL. The method was precise with intra-day relative standard deviation (RSD) of lower than 1% and 2.06% for inter-day RSD. The method accuracy represented by percent recover was 99.58%. The highly efficient HPLC system developed from this study could detect morin contents in M. cochinchinensis heartwood samples collected from various locations in Thailand in the range of 0.74-1.57% w/w. This developed method provided a useful standardization procedure of M. cochincihinesis materials for further application in pharmacy and other commercial developments.
已开发出高效液相色谱 - 二极管阵列检测法（HPLC - DAD）来测定黄槿（Maclura cochinchinensis Corner）心材提取物中的桑色素含量。色谱分离采用Hypersil BDS C18柱，以水:乙腈（80:20）中含0.5%乙酸的等度溶剂系统，流速为1.0 mL/min，并在355 nm处进行检测。桑色素的标准曲线在7 - 905 μg/mL范围内呈线性。该方法精密度良好，日内相对标准偏差（RSD）低于1%，日间RSD为2.06%。以回收率表示的方法准确度为99.58%。本研究开发的高效HPLC系统能够检测从泰国不同地点采集的黄槿心材样品中的桑色素含量，范围为0.74 - 1.57%（w/w）。该开发方法为黄槿材料在药学及其他商业开发中的进一步应用提供了有用的标准化程序。
