一种用于定量分析心材中桑色素的经验证的高效薄层色谱法。
A validated HPTLC method for quantitative analysis of morin in heartwood.
作者信息
Chewchinda Savita, Kongkiatpaiboon Sumet
机构信息
Department of Food Chemistry, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand.
Drug Discovery and Development Center, Office of Advanced Science and Technology, Thammasat University (Rangsit Campus), Pathum Thani 12121, Thailand.
出版信息
Chin Herb Med. 2020 Mar 18;12(2):200-203. doi: 10.1016/j.chmed.2019.09.008. eCollection 2020 Apr.
OBJECTIVE
The study was conducted to develop and validate a high performance thin-layer chromatography (HPTLC)-densitometric method for the quantitative analysis of morin in heartwood collected from different locations in Thailand.
METHODS
HPTLC analysis was performed on an aluminium sheet of silica gel 60 F254 using toluene: ethyl acetate: formic acid (36:12:7, volume percent) as a mobile phase. The densitometric scanning was performed at the wavelength 410 nm. HPTLC method was validated according to ICH guideline.
RESULTS
The proposed HPTLC method showed acceptable validation parameters. The content of morin in heartwood collected from eight different provinces in Thailand were in the ranges of 1.53%-2.73%.
CONCLUSION
The simple and sensitive HPTLC method was successfully developed and validated for determination of morin in heartwood. The proposed HPTLC method was found to be simple, fast and inexpensive, and can be used for the routine quality control of raw materials.
目的
开展并验证一种高效薄层色谱（HPTLC）-密度测定法，用于定量分析从泰国不同地点采集的心材中桑色素的含量。
方法
使用硅胶60 F254铝板进行HPTLC分析，以甲苯：乙酸乙酯：甲酸（36:12:7，体积百分比）作为流动相。在波长410 nm处进行密度扫描。HPTLC方法按照国际人用药品注册技术协调会（ICH）指南进行验证。
结果
所提出的HPTLC方法显示出可接受的验证参数。从泰国八个不同省份采集的心材中桑色素的含量在1.53%-2.73%范围内。
结论
成功开发并验证了一种简单灵敏的HPTLC方法用于测定心材中的桑色素。所提出的HPTLC方法简单、快速且成本低廉，可用于原材料的常规质量控制。
Zotero 插件