Department of Biology and Biotechnology, AG Photobiotechnology, Ruhr-University Bochum, Universitätsstraße 150, 44801, Bochum, Germany.
Department of Chemistry and Biochemistry, Ruhr-University Bochum, Chair of Inorganic Chemistry I, Universitätsstraße 150, 44801, Bochum, Germany.
ChemSusChem. 2017 Mar 9;10(5):894-902. doi: 10.1002/cssc.201601523. Epub 2017 Feb 9.
We report a sustainable in vitro system for enzyme-based photohydrogen production. The [FeFe]-hydrogenase HydA1 from Chlamydomonas reinhardtii was tested for photohydrogen production as a proton-reducing catalyst in combination with eight different photosensitizers. Using the organic dye 5-carboxyeosin as a photosensitizer and plant-type ferredoxin PetF as an electron mediator, HydA1 achieves the highest light-driven turnover number (TON ) yet reported for an enzyme-based in vitro system (2.9×10 mol(H ) mol(cat) ) and a maximum turnover frequency (TOF ) of 550 mol(H ) mol(HydA1) s . The system is fueled very effectively by ambient daylight and can be further simplified by using 5-carboxyeosin and HydA1 as a two-component photosensitizer/biocatalyst system without an additional redox mediator.
我们报告了一种基于酶的光氢生产的可持续体外系统。将莱茵衣藻的[FeFe]-氢化酶 HydA1 作为质子还原催化剂进行了光氢生产测试，与八种不同的光敏剂结合使用。使用有机染料 5-羧基荧光素作为光敏剂和植物型铁氧还蛋白 PetF 作为电子介体，HydA1 实现了基于酶的体外系统中报道的最高光驱动周转数（TON）（2.9×10 mol(H) mol(cat)）和最高周转频率（TOF）（550 mol(H) mol(HydA1) s-1）。该系统可以非常有效地利用环境日光进行供能，并且可以通过使用 5-羧基荧光素和 HydA1 作为无外加氧化还原介体的两组件光敏剂/生物催化剂系统进一步简化。
