Otaguiri Eliane S, Morguette Ana E B, Biasi-Garbin Renata P, Morey Alexandre T, Lancheros Cesar A C, Kian Danielle, de Oliveira Admilton G, Kerbauy Gilselena, Perugini Marcia R E, Duran Nelson, Nakamura Celso V, da Veiga Valdir F, Nakazato Gerson, Pinge-Filho Phileno, Yamauchi Lucy M, Yamada-Ogatta Sueli F
Departamento de Microbiologia, Centro de Ciencias Biologicas, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, PR 445, km 380, CEP: 86057970, Londrina, Parana, Brazil.
Curr Pharm Biotechnol. 2017;18(2):177-190. doi: 10.2174/1389201017666161213151919.
Streptococcus agalactiae (group B Streptococcus - GBS) remains a leading cause of neonatal infections and an important cause of invasive infections in adults with underlying conditions.
This study evaluated for the first time the effect of an oleoresin collected from Copaifera multijuga Hayne (copaiba oil) alone or in combination with silver nanoparticles produced by green synthesis using Fusarium oxysporum (AgNPbio) against planktonic and sessile cells of GBS isolated from colonized women.
Copaiba oil showed a dose-dependent bactericidal activity against planktonic GBS strains, including those resistant to erythromycin and/or clindamycin. Scanning and transmission electron microscopy of GBS treated with copaiba oil revealed morphological and ultrastructural alterations, displaying disruption of the cell wall and decreased electron density due to leakage of cytoplasmic content. Copaiba oil also exhibited antibacterial activity against biofilms of GBS strains, inhibiting their formation as well as the viability of mature biofilms. In addition, the combination of copaiba oil with AgNPbio resulted in a synergistic effect against planktonic cells and biofilm formation, reducing the minimal inhibitory concentration values of both compounds. No hemolytic activity was detected for both compounds.
These results indicate the potential of copaiba oil, alone or in combination with AgNPbio, for the development of new alternative strategies for controlling GBS infections.
无乳链球菌（B族链球菌 - GBS）仍然是新生儿感染的主要原因，也是有基础疾病成人侵袭性感染的重要原因。
本研究首次评估了从多脂苦配巴（苦配巴油）中提取的油树脂单独使用或与尖孢镰刀菌绿色合成产生的银纳米颗粒（AgNPbio）联合使用对从定植女性中分离出的GBS浮游细胞和固着细胞的影响。
苦配巴油对GBS浮游菌株显示出剂量依赖性杀菌活性，包括对红霉素和/或克林霉素耐药的菌株。用苦配巴油处理的GBS的扫描和透射电子显微镜显示出形态和超微结构改变，表现为细胞壁破坏以及由于细胞质内容物泄漏导致电子密度降低。苦配巴油对GBS菌株的生物膜也表现出抗菌活性，抑制其形成以及成熟生物膜的活力。此外，苦配巴油与AgNPbio的组合对浮游细胞和生物膜形成产生协同作用，降低了两种化合物的最低抑菌浓度值。两种化合物均未检测到溶血活性。
这些结果表明苦配巴油单独或与AgNPbio联合使用在开发控制GBS感染的新替代策略方面具有潜力。
