Yalçınkaya Burhanettin, Yumbul Eylem, Mozioğlu Erkan, Akgoz Muslum
Bioanalysis Laboratory, TUBITAK National Metrology Institute (UME), Gebze, Kocaeli, Turkey; Department of Molecular Medicine, Institute for Experimental Medical Research, Istanbul University, Istanbul, Turkey.
Bioanalysis Laboratory, TUBITAK National Metrology Institute (UME), Gebze, Kocaeli, Turkey; Department of Molecular Biology and Genetics, Atatürk University, Erzurum, Turkey.
Food Chem. 2017 Apr 15;221:1253-1257. doi: 10.1016/j.foodchem.2016.11.032. Epub 2016 Nov 9.
Preventing adulteration of meat and meat products with less desirable or objectionable meat species is important not only for economical, religious and health reasons, but also, it is important for fair trade practices, therefore, several methods for identification of meat and meat products have been developed. In the present study, ten different DNA extraction methods, including Tris-EDTA Method, a modified Cetyltrimethylammonium Bromide (CTAB) Method, Alkaline Method, Urea Method, Salt Method, Guanidinium Isothiocyanate (GuSCN) Method, Wizard Method, Qiagen Method, Zymogen Method and Genespin Method were examined to determine their relative effectiveness for extracting DNA from meat samples. The results show that the salt method is easy to perform, inexpensive and environmentally friendly. Additionally, it has the highest yield among all the isolation methods tested. We suggest this method as an alternative method for DNA isolation from meat and meat products.
防止用不太理想或令人反感的肉类品种掺假肉类和肉类产品不仅出于经济、宗教和健康原因很重要，而且对于公平贸易做法也很重要。因此，已经开发了几种鉴定肉类和肉类产品的方法。在本研究中，研究了十种不同的DNA提取方法，包括三羟甲基氨基甲烷-乙二胺四乙酸（Tris-EDTA）法、改良的十六烷基三甲基溴化铵（CTAB）法、碱法、尿素法、盐法、异硫氰酸胍（GuSCN）法、Wizard法、Qiagen法、酶原法和Genespin法，以确定它们从肉类样品中提取DNA的相对有效性。结果表明，盐法操作简便、成本低廉且环保。此外，在所有测试分离方法中它的产量最高。我们建议将此方法作为从肉类和肉类产品中分离DNA的替代方法。
