He Bo, Ge Jiao, Yue Pengxiang, Yue XueYang, Fu Ruiyan, Liang Jin, Gao Xueling
College of Tea & Food Science, Anhui Agricultural University, Hefei 230036, People's Republic of China.
Fujian Provincial Key Laboratory for Extracting & Processing Technology of Edible Plant, Zhangzhou, Fujian, People's Republic of China.
Food Chem. 2017 Apr 15;221:1671-1677. doi: 10.1016/j.foodchem.2016.10.120. Epub 2016 Nov 1.
The optimal preparation parameters to create anthocyanin-loaded chitosan nanoparticles was predicted using response surface methodology (RSM). A Box-Behnken design was used to determine the preparation parameters that would achieve the preferred particle size and high encapsulation efficiency. The result suggested that the optimized conditions were 2.86mg/mL carboxymethyl chitosan (CMC), 0.98mg/mL chitosan hydrochloride (CHC) and 5.97mg anthocyanins. Using the predicted amounts, the experimentally prepared particles averaged 219.53nm with 63.15% encapsulation efficiency. The result was less than 5% different than the predicted result of 214.83nm particle size and 61.80% encapsulation efficiency. Compared with the free anthocyanin solution, the anthocyanin-loaded chitosan nanoparticles showed a slowed degradation in simulated gastrointestinal fluid. Compared with the free anthocyanin solutions in a model beverage system, the stability of the anthocyanins was increased in the anthocyanin-loaded chitosan nanoparticles.
采用响应面法（RSM）预测了制备负载花青素的壳聚糖纳米粒的最佳制备参数。采用Box-Behnken设计来确定能够实现优选粒径和高包封率的制备参数。结果表明，优化条件为2.86mg/mL羧甲基壳聚糖（CMC）、0.98mg/mL盐酸壳聚糖（CHC）和5.97mg花青素。使用预测的量，实验制备的颗粒平均粒径为219.53nm，包封率为63.15%。该结果与预测的粒径214.83nm和包封率61.80%的结果相差不到5%。与游离花青素溶液相比，负载花青素的壳聚糖纳米粒在模拟胃肠液中的降解速度减慢。与模型饮料系统中的游离花青素溶液相比，负载花青素的壳聚糖纳米粒中花青素的稳定性有所提高。
