Department of Oncology, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, China.
Department of Respiration, The Affiliated Jiangning Hospital of Nanjing Medical University, Nanjing, 210000, China.
Cancer Med. 2018 Jun;7(6):2370-2381. doi: 10.1002/cam4.1490. Epub 2018 Apr 19.
Dysregulated microRNAs (miRNAs) in the plasma of patients with lung squamous cell carcinoma (LSCC) might serve as biomarkers for LSCC diagnosis. The expression of miRNAs was performed using quantitative reverse transcription-polymerase chain reaction (qRT-PCR) on the basis of Exiqon panels in the initial screening phase including three male LSCC pool samples and one normal control (NC) pool sample (per 10 samples were pooled as one pool sample). After the training (32 LSCC vs. 31 NCs), the testing (55 LSCC vs. 55 NCs), and the external validation (15 LSCC vs. 15 NCs) stages via qRT-PCR, a four-miRNA signature (miR-181a-5p, miR-21-5p, miR-106a-5p, and miR-93-5p) was identified for LSCC detection. Areas under the receiver operating characteristic (ROC) curve (AUC) of the four-miRNA panel for the training, the testing, and the external validation phases were 0.795, 0.827, and 0.914, respectively. Then, the four miRNAs were explored in LSCC tissue samples (23 LSCC vs. 23 NCs), and their expression was significantly up-regulated. However, none of the four miRNAs found significantly up-regulated in plasma exosomes expect miR-93-5p with borderline significance (16 LSCC vs. 16 NCs). In summary, our study established a four-miRNA peripheral plasma signature, which contributed to diagnosing male LSCC patients in China to a certain degree.
在肺鳞状细胞癌（LSCC）患者的血浆中失调的 microRNAs（miRNAs）可能作为 LSCC 诊断的生物标志物。在初始筛选阶段，根据 Exiqon 面板使用定量逆转录聚合酶链反应（qRT-PCR）进行 miRNA 的表达，包括三个男性 LSCC 池样本和一个正常对照（NC）池样本（每 10 个样本混合为一个池样本）。经过 qRT-PCR 的训练（32 例 LSCC 与 31 例 NC）、测试（55 例 LSCC 与 55 例 NC）和外部验证（15 例 LSCC 与 15 例 NC）阶段后，确定了用于 LSCC 检测的四 miRNA 特征（miR-181a-5p、miR-21-5p、miR-106a-5p 和 miR-93-5p）。该四 miRNA 面板在训练、测试和外部验证阶段的接收者操作特征（ROC）曲线下面积（AUC）分别为 0.795、0.827 和 0.914。然后，在 LSCC 组织样本（23 例 LSCC 与 23 例 NC）中探索了这四个 miRNA，发现它们的表达显著上调。然而，除 miR-93-5p 外，没有一个 miRNA 在血浆外泌体中显著上调，而 miR-93-5p 具有边缘显著性（16 例 LSCC 与 16 例 NC）。总之，我们的研究建立了一个四 miRNA 外周血浆特征，在一定程度上有助于诊断中国男性 LSCC 患者。
