Department of Pathology, School of Basic Medical Sciences, Xiangya HospitalCentral South UniversityHunan Province, Changsha, 410000, China.
Guangzhou Key Laboratory of Formula-Pattern of Traditional Chinese Medicine, School of Traditional Chinese Medicine, Jinan University, Guangzhou, 510632, Guangdong, People's Republic of China.
Cell Mol Life Sci. 2022 Apr 22;79(5):254. doi: 10.1007/s00018-022-04250-0.
Previous studies in our laboratory have reported that miR-222-3p was a tumor-suppressive miRNA in OC. This study aims to further understand the regulatory role of miR-222-3p in OC and provide a new mechanism for its prevention and treatment. We first found that miR-222-3p inhibited the migration and proliferation of OC cells. Then, we observed CDK19 was highly expressed in OC and inversely correlated with miR-222-3p. Besides, we observed that miR-222-3p directly binds to the 3'-UTR of CDK19 and inhibits CDK19 translation, thus inhibiting OC cell migration and proliferation in vitro and repressed tumor growth in vivo. We also observed the inhibitory effect of Hotair on miR-222-3p in OC. In addition, Hotair could promote the proliferation and migration of OC cells in vitro and facilitate the growth and metastasis of tumors in vivo. Moreover, Hotair was positively correlated with CDK19 expression. These results suggest Hotair indirectly up-regulates CDK19 through sponging miR-222-3p, which enhances the malignant behavior of OC. This provides a further understanding of the mechanism of the occurrence and development of OC.
先前在我们实验室进行的研究表明，miR-222-3p 是 OC 中的一种肿瘤抑制 miRNA。本研究旨在进一步了解 miR-222-3p 在 OC 中的调控作用，并为其预防和治疗提供新的机制。我们首先发现 miR-222-3p 抑制 OC 细胞的迁移和增殖。然后，我们观察到 CDK19 在 OC 中高表达，并且与 miR-222-3p 呈负相关。此外，我们观察到 miR-222-3p 可直接结合 CDK19 的 3'-UTR 并抑制 CDK19 的翻译，从而抑制 OC 细胞的迁移和增殖。我们还观察到 Hotair 在 OC 中对 miR-222-3p 的抑制作用。此外，Hotair 可促进 OC 细胞在体外的增殖和迁移，并促进体内肿瘤的生长和转移。此外，Hotair 与 CDK19 的表达呈正相关。这些结果表明 Hotair 通过海绵吸附 miR-222-3p 间接上调 CDK19，从而增强 OC 的恶性行为。这为进一步了解 OC 的发生和发展机制提供了依据。
