Bal Natalia V, Rysakova Mariia P, Vinarskaya Alia Kh, Ivanova Violetta, Zuzina Alena B, Balaban Pavel M
Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, 5A Butlerova St., Moscow, 117485, Russia.
Eur J Neurosci. 2017 Mar;45(5):643-647. doi: 10.1111/ejn.13503. Epub 2017 Jan 9.
It is well-known that the reactivation of consolidated fear memory under boundary conditions of novelty and protein synthesis blockade results in an impairment of memory, suggesting that the reactivated memory is destabilized and requires synthesis of new proteins for reconsolidation. We tested the hypothesis of nitric oxide (NO) involvement in memory destabilization during the reconsolidation process in rats using memory reactivation under different conditions. We report that administration of NO-synthase selective blockers 3-Br-7-NI or ARL in the conditions of reactivation of memory under a protein synthesis blockade prevented destabilization of fear memory to the conditioned stimulus. Obtained results support the role of NO signaling pathway in the destabilization of existing fear memory triggered by reactivation, and demonstrate that the disruption of this pathway during memory reconsolidation may prevent changes in long-term memory.
众所周知，在新奇性和蛋白质合成阻断的边界条件下，巩固的恐惧记忆重新激活会导致记忆受损，这表明重新激活的记忆会变得不稳定，需要合成新的蛋白质进行重新巩固。我们使用不同条件下的记忆重新激活，测试了一氧化氮（NO）参与大鼠重新巩固过程中记忆不稳定的假设。我们报告，在蛋白质合成阻断条件下记忆重新激活时，给予NO合酶选择性阻滞剂3-Br-7-NI或ARL可防止恐惧记忆对条件刺激的不稳定。获得的结果支持NO信号通路在由重新激活引发的现有恐惧记忆不稳定中的作用，并表明在记忆重新巩固过程中该通路的破坏可能会阻止长期记忆的变化。
