Sichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu, Sichuan, 610051, PR China.
Department of Veterinary Medicine, Southwest University, Rongchang, Chongqing 402460, PR China.
Carbohydr Polym. 2017 Feb 10;157:629-636. doi: 10.1016/j.carbpol.2016.10.040. Epub 2016 Oct 14.
The water-soluble polysaccharides were extracted and purified from the root of Chuanminshen violaceum (CVPS). The antioxidant activities of the CVPS were evaluated both with in vitro and in vivo experiments. The results of the in vitro antioxidant assay suggested that the CVPS scavenged DPPH, hydroxyl, and superoxide anion radicals. The oral administration of three different doses of CVPS administered over a period of 6 weeks to D-galactose induced aging mice models, enhanced the activities of T-SOD, Mn-SOD, Cu, Zn-SOD, and CAT, and markedly decreased the content of MDA. Therefore, significant up-regulation of mRNA expression levels of Cu, Zn-SOD, Mn-SOD, CAT, glutathione peroxidase 1 (GPx), thioredoxin 1 (Trx1), and thioredoxin 2 (Trx1) occurred. Finally, the results demonstrated that the CVPS are a novel potential resource for natural antioxidants and anti-aging drugs.
从川明参根部分离提取出水溶性多糖，并对其进行了纯化。采用体外和体内实验评估了川明参的抗氧化活性。体外抗氧化试验结果表明，川明参可以清除 DPPH、羟自由基和超氧阴离子自由基。通过灌胃方式给予不同剂量的川明参，持续 6 周，对 D-半乳糖诱导的衰老小鼠模型进行处理，结果表明川明参能够提高 T-SOD、Mn-SOD、Cu、Zn-SOD 和 CAT 的活性，同时显著降低 MDA 的含量。因此，Cu、Zn-SOD、Mn-SOD、CAT、谷胱甘肽过氧化物酶 1（GPx）、硫氧还蛋白 1（Trx1）和硫氧还蛋白 2（Trx2）的 mRNA 表达水平显著上调。最后，研究结果表明，川明参是一种具有开发潜力的天然抗氧化剂和抗衰老药物资源。
