miR-151a 通过调节白细胞介素-12 受体 β2 参与特应性皮炎的发病机制。

MiR-151a is involved in the pathogenesis of atopic dermatitis by regulating interleukin-12 receptor β2.

机构信息

Shenzhen Key Laboratory for Translational Medicine of Dermatology, Biomedical Research Institute, Shenzhen Peking University - The Hong Kong University of Science and Technology Medical Center, Shenzhen, Guangdong Province, China.

Department of Dermatology, Peking University Shenzhen Hospital, Shenzhen, Guangdong Province, China.

出版信息

Exp Dermatol. 2018 Apr;27(4):427-432. doi: 10.1111/exd.13276. Epub 2017 Apr 11.

DOI:10.1111/exd.13276

PMID:27992076

Abstract

MicroRNAs (miRNAs) have been reported to circulate in the blood in a highly stable and cell-free form. Dysregulated expression of miRNAs has been detected in various pathological conditions including atopic dermatitis. In our study, human blood plasma miRNAs were identified by high-throughput sequencing and compared among patients of atopic dermatitis and healthy controls. We found that miR-151a was differentially expressed in the plasma of atopic dermatitis patients. MiR-151a regulates the expression of IL12RB2 by targeting two loci in the 3' untranslated region of the Il12rb2 gene. Moreover, IL12RB2 was remarkably downregulated in Jurkat cells overexpressing miR-151a. Jurkat cells treated with phytohemagglutinin also showed reduced expression of IFN-γ, interleukin-2 (IL-2) and IL-12. Together, these results suggest that miR-151a is involved in the pathogenesis of atopic dermatitis by regulating IL12RB2.

摘要

MicroRNAs (miRNAs) 已被报道以高度稳定和无细胞的形式在血液中循环。在各种病理情况下，包括特应性皮炎，都检测到 miRNA 的表达失调。在我们的研究中，通过高通量测序鉴定了人血浆中的 miRNAs，并在特应性皮炎患者和健康对照组之间进行了比较。我们发现 miR-151a 在特应性皮炎患者的血浆中表达差异。miR-151a 通过靶向 Il12rb2 基因 3'非翻译区的两个位点调节 IL12RB2 的表达。此外，在过表达 miR-151a 的 Jurkat 细胞中，IL12RB2 显著下调。用植物血凝素处理的 Jurkat 细胞也表现出 IFN-γ、白细胞介素-2 (IL-2) 和 IL-12 的表达减少。综上所述，这些结果表明 miR-151a 通过调节 IL12RB2 参与特应性皮炎的发病机制。