Butin M, Martins-Simões P, Pichon B, Leyssene D, Bordes-Couecou S, Meugnier H, Rouard C, Lemaitre N, Schramm F, Kearns A, Spiliopoulou I, Hyyryläinen H-L, Dumitrescu O, Vandenesch F, Dupieux C, Laurent F
International Center for Infectiology Research (CIRI), INSERM U1111-CNRS UMR5308-ENS Lyon-University Claude Bernard Lyon 1, France.
Department of Bacteriology, Hospices Civils de Lyon, Lyon, France.
J Antimicrob Chemother. 2017 Apr 1;72(4):1014-1020. doi: 10.1093/jac/dkw516.
We investigated the epidemiological, clinical, microbiological and genetic characteristics of linezolid-resistant (LZR) Staphylococcus capitis isolates from French ICUs, and compared them with LZR S. capitis isolates from other European countries.
All LZR isolates were subjected to antimicrobial susceptibility testing (AST) and the presence of cfr and optrA genes as well as mutations in the 23S rRNA and ribosomal proteins were investigated using specific PCR with sequencing. The genetic relationship between isolates was investigated using PFGE and WGS. Epidemiological data concerning LZR S. capitis were collected retrospectively in French microbiology laboratories.
Twenty-one LZR isolates were studied: 9 from France, 11 from Greece and 1 from Finland. All were resistant to methicillin and aminoglycosides. In addition, this unusual AST profile was identified in S. capitis isolates from seven French hospitals, and represented up to 12% of the S. capitis isolates in one centre. A G2576T mutation in 23S rRNA was identified in all isolates; cfr and optrA genes were absent. All isolates belonged to the same clone on the basis of their PFGE profiles, whatever their geographical origin. WGS found at most 212 SNPs between core genomes of the LZR isolates.
We identified and characterized an LZR S. capitis clone disseminated in three European countries, harbouring the same multiple resistance and a G2576T mutation in the 23S rRNA. The possible unrecognized wider distribution of this clone, belonging to a species classically regarded as a low-virulence skin colonizer, is of major concern not least because of the increasing use of oxazolidinones.
我们调查了来自法国重症监护病房（ICU）的耐利奈唑胺（LZR）头状葡萄球菌分离株的流行病学、临床、微生物学和遗传学特征，并将它们与来自其他欧洲国家的耐利奈唑胺头状葡萄球菌分离株进行比较。
对所有耐利奈唑胺分离株进行抗菌药物敏感性试验（AST），并使用特异性PCR及测序研究cfr和optrA基因的存在情况以及23S rRNA和核糖体蛋白中的突变。使用脉冲场凝胶电泳（PFGE）和全基因组测序（WGS）研究分离株之间的遗传关系。关于耐利奈唑胺头状葡萄球菌的流行病学数据在法国微生物实验室进行了回顾性收集。
研究了21株耐利奈唑胺分离株：9株来自法国，11株来自希腊，1株来自芬兰。所有分离株均对甲氧西林和氨基糖苷类耐药。此外，在来自七家法国医院的头状葡萄球菌分离株中发现了这种不寻常的AST谱型，在一个中心占头状葡萄球菌分离株的比例高达12%。在所有分离株中均鉴定出23S rRNA中的G2576T突变；未发现cfr和optrA基因。无论地理来源如何，根据PFGE谱型，所有分离株均属于同一克隆。WGS发现耐利奈唑胺分离株的核心基因组之间最多有212个单核苷酸多态性（SNP）。
我们鉴定并描述了一个在三个欧洲国家传播的耐利奈唑胺头状葡萄球菌克隆，其具有相同的多重耐药性且23S rRNA中有G2576T突变。鉴于恶唑烷酮类药物的使用日益增加，这个属于通常被认为是低毒力皮肤定植菌的物种的克隆可能存在未被认识到的更广泛分布，这是一个主要问题。
