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茄子花叶芜菁黄花叶病毒基因组的核苷酸序列

Nucleotide sequence of the genome of eggplant mosaic tymovirus.

作者信息

Osorio-Keese M E, Keese P, Gibbs A

机构信息

Research School of Biological Sciences, Australian National University, Canberra.

出版信息

Virology. 1989 Oct;172(2):547-54. doi: 10.1016/0042-6822(89)90197-9.

DOI:10.1016/0042-6822(89)90197-9
PMID:2800336
Abstract

The sequence of the RNA genome of an isolate of eggplant mosaic tymovirus from Trinidad (EMV-Trin) has been determined. The genome is 6330 nucleotide residues in length and contains three open reading frames; two overlapping genes, whose initiation codons are separated by seven nucleotide residues (nucleotide residues 102-2051 and 109-5628) near the 5' terminus, and the virion protein gene, which is near the 3' terminus (nucleotide residues 5633-6199). The genomes of EMV-Trin and turnip yellow mosaic tymovirus have the same genomic organization and similar nucleotide and encoded amino acid sequences. The nucleotide residues adjacent to the initiation codons of tymoviral overlapping genes have closely similar sequences which may form a weak stem-loop secondary structure that regulates their translation.

摘要

已测定了来自特立尼达的茄子花叶番茄病毒分离株(EMV-Trin)的RNA基因组序列。该基因组长度为6330个核苷酸残基,包含三个开放阅读框;两个重叠基因,其起始密码子在5'末端附近被七个核苷酸残基(核苷酸残基102 - 2051和109 - 5628)隔开,以及位于3'末端附近的病毒粒子蛋白基因(核苷酸残基5633 - 6199)。EMV-Trin和芜菁黄花叶番茄病毒的基因组具有相同的基因组组织以及相似的核苷酸和编码氨基酸序列。番茄病毒重叠基因起始密码子相邻的核苷酸残基具有非常相似的序列,可能形成一个弱的茎环二级结构来调节它们的翻译。

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Protonatable hairpins are conserved in the 5'-untranslated region of tymovirus RNAs.
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