Lai Bai-Min, Zhang Kun, Shen Dong-Sheng, Wang Mei-Zhen, Shentu Jia-Li, Li Na
School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou, 310012, People's Republic of China.
Zhejiang Provincial Key Laboratory of Solid Waste Treatment and Recycling, Hangzhou, 310012, People's Republic of China.
Environ Sci Pollut Res Int. 2017 Feb;24(6):5259-5267. doi: 10.1007/s11356-016-8260-2. Epub 2016 Dec 21.
To investigate whether pollution from antibiotic resistance genes (ARGs) could be affected by bacterial quorum sensing, the oxytetracycline (OTC)-containing manure was fertilized to establish the ARG-polluted soil environment. Under long-term OTC stress, substantial ARGs in the range from 10 to 10 RG/16S rRNA (resistance genes/16S rRNA) were detected in the antibiotics control (AC) group, in which OTC-containing manure was fertilized. Meanwhile, 10 RG/16S rRNA was detected in biological control (BC) group, in which non-OTC-containing manure was fertilized. Subsequently, two typical quorum sensing inhibitors, 4-nitropyridine N-oxide (4-NPO) and 3,4-dibromo-2H-furan-5-one (DBF), were used to treat the ARG-polluted soils. These two groups called 4-NPO treatments (NT) and DBF treatments (FT), respectively. There were no significant differences in bacterial growth and OTC degradation in NT and FT groups, compared to AC group. However, acyl-homoserine lactones such as C-HSL, C-HSL, and C-HSL decreased significantly in both NT and FT groups, compared to AC group. Meanwhile, the abundance of most ARGs decreased dramatically. In FT group, the concentrations of tet(L) and tet(Q) were below the detection limits. It was demonstrated that quorum sensing inhibition could be an effective way to prevent and control the pollution of ARGs in soil.
为了研究抗生素抗性基因（ARGs）污染是否会受到细菌群体感应的影响，向土壤中施加含土霉素（OTC）的粪肥以建立受ARGs污染的土壤环境。在长期OTC胁迫下，在施加含OTC粪肥的抗生素对照组（AC组）中检测到大量ARGs，范围为10至10⁶抗性基因/16S rRNA（抗性基因/16S rRNA）。同时，在施加不含OTC粪肥的生物对照组（BC组）中检测到10³抗性基因/16S rRNA。随后，使用两种典型的群体感应抑制剂4-硝基吡啶N-氧化物（4-NPO）和3,4-二溴-2H-呋喃-5-酮（DBF）处理受ARGs污染的土壤。这两组分别称为4-NPO处理组（NT组）和DBF处理组（FT组）。与AC组相比，NT组和FT组的细菌生长和OTC降解没有显著差异。然而，与AC组相比，NT组和FT组中的酰基高丝氨酸内酯如C₄-HSL、C₈-HSL和C₁₂-HSL显著降低。同时，大多数ARGs的丰度显著下降。在FT组中，tet(L)和tet(Q)的浓度低于检测限。结果表明，抑制群体感应可能是预防和控制土壤中ARGs污染的有效方法。
