CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology , Beijing 100190, P. R. China.
University of Chinese Academy of Sciences , Beijing 100049, P. R. China.
ACS Appl Mater Interfaces. 2017 Jan 18;9(2):1226-1236. doi: 10.1021/acsami.6b11209. Epub 2017 Jan 6.
The integration of different therapies into a single nanoplatform has shown great promise for synergistic tumor treatment. Herein, mesoporous silica (MS) coated polydopamine functionalized reduced graphene oxide (pRGO) further modified with hyaluronic acid (HA) (pRGO@MS-HA) has been utilized as a versatile nanoplatform for synergistic targeted chemo-photothermal therapy against cancer. A facile and green chemical method is adopted for the simultaneous reduction and noncovalent functionalization of graphene oxide (GO) by using mussel inspired dopamine (DA) to enhance biocompatibility and the photothermal effect. Then, it was coated with mesoporous silica (MS) (pRGO@MS) to enhance doxorubicin (DOX) loading and be further modified with the targeting moieties hyaluronic acid (HA). The pH-dependent and near-infrared (NIR) laser irradiation-triggered DOX release from pRGO@MS(DOX)-HA is observed, which could enhance the chemo-photothermal therapy effect. In vitro experimental results confirm that pRGO@MS(DOX)-HA exhibits good dispersibility, excellent photothermal property, remarkable tumor cell killing efficiency, and specificity to target tumor cells. In vivo antitumor experiments further demonstrated that pRGO@MS(DOX)-HA could exhibit an excellent synergistic antitumor efficacy, which is much more distinct than any monotherapy. This work presents a novel nanoplatform which could load chemotherapy drugs with high efficiency and be used as light-mediated photothermal cancer therapy agent.
不同疗法的整合到单个纳米平台中已经显示出协同肿瘤治疗的巨大前景。在此，介孔硅（MS）包覆的聚多巴胺功能化还原氧化石墨烯（pRGO）进一步用透明质酸（HA）修饰（pRGO@MS-HA）被用作协同靶向化疗-光热治疗癌症的多功能纳米平台。采用简便、绿色的化学方法，通过贻贝启发的多巴胺（DA）同时还原和非共价功能化氧化石墨烯（GO），以提高生物相容性和光热效应。然后，用介孔硅（MS）包覆（pRGO@MS），以增强阿霉素（DOX）的负载，并进一步用靶向部分透明质酸（HA）修饰。观察到 pRGO@MS（DOX）-HA 的 pH 依赖性和近红外（NIR）激光照射触发的 DOX 释放，这可以增强化疗-光热治疗效果。体外实验结果证实，pRGO@MS（DOX）-HA 具有良好的分散性、优异的光热性能、显著的肿瘤细胞杀伤效率以及对靶肿瘤细胞的特异性。体内抗肿瘤实验进一步证明，pRGO@MS（DOX）-HA 可以表现出优异的协同抗肿瘤疗效，明显优于任何单一疗法。这项工作提出了一种新型纳米平台，可高效负载化疗药物，并用作光介导的光热癌症治疗剂。
