Zhao Yulong, Wang Yankai, Yang Hao, Wang Wei, Wu Jianyu, Hu Xiuli
State Key Laboratory of Wheat and Maize Crop Science, Collaborative Innovation Center of Henan Grain Crops, Henan Agricultural University Zhengzhou, China.
Front Plant Sci. 2016 Dec 8;7:1827. doi: 10.3389/fpls.2016.01827. eCollection 2016.
Drought stress is one of major factors resulting in maize yield loss. The roles of abscisic acid (ABA) have been widely studied in crops in response to drought stress. However, more attention is needed to identify key ABA-related proteins and also gain deeper molecular insights about drought stress in maize. Based on this need, the physiology and proteomics of the ABA-deficient maize mutant and its wild-type under drought stress were examined and analyzed. Malondialdehyde content increased and quantum efficiency of photosystem II decreased under drought stress in both genotypes. However, the magnitude of the increase or decrease was significantly higher in than in . A total of 7051 proteins with overlapping expression patterns among three replicates in the two genotypes were identified by Multiplex run iTRAQ-based quantitative proteomic and liquid chromatography-tandem mass spectrometry methods, of which the expression of only 150 proteins (130 in , 27 in ) showed changes of at least 1.5-fold under drought stress. Among the 150 proteins, 67 and 60 proteins were up-regulated and down-regulated by drought stress in an ABA-dependent way, respectively. ABA was found to play active roles in regulating signaling pathways related to photosynthesis, oxidative phosphorylation (mainly related to ATP synthesis), and glutathione metabolism (involved in antioxidative reaction) in the maize response to drought stress. Our results provide an extensive dataset of ABA-dependent, drought-regulated proteins in maize plants, which may help to elucidate the underlying mechanisms of ABA-enhanced tolerance to drought stress in maize.
干旱胁迫是导致玉米产量损失的主要因素之一。脱落酸（ABA）在作物应对干旱胁迫中的作用已得到广泛研究。然而，需要更多关注来鉴定关键的ABA相关蛋白，并更深入地了解玉米干旱胁迫的分子机制。基于此需求，对ABA缺陷型玉米突变体及其野生型在干旱胁迫下的生理和蛋白质组学进行了检测和分析。在干旱胁迫下，两种基因型的丙二醛含量均增加，光系统II的量子效率均降低。然而，[突变体]中增加或降低的幅度明显高于[野生型]。通过基于多重运行iTRAQ的定量蛋白质组学和液相色谱 - 串联质谱方法，在两种基因型的三个重复样本中鉴定出总共7051种具有重叠表达模式的蛋白质，其中只有150种蛋白质（[突变体]中130种，[野生型]中27种）的表达在干旱胁迫下显示出至少1.5倍的变化。在这150种蛋白质中，分别有67种和60种蛋白质在干旱胁迫下以ABA依赖的方式上调和下调。发现ABA在调节玉米对干旱胁迫响应中与光合作用、氧化磷酸化（主要与ATP合成有关）和谷胱甘肽代谢（参与抗氧化反应）相关的信号通路中发挥积极作用。我们的结果提供了玉米植株中ABA依赖的、干旱调节蛋白的广泛数据集，这可能有助于阐明ABA增强玉米对干旱胁迫耐受性的潜在机制。
