College of Agronomy, Henan Agricultural University, Zhengzhou, China.
State Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou, China.
Sci Rep. 2019 Aug 13;9(1):11741. doi: 10.1038/s41598-019-47926-7.
Plant roots are vital for acquiring nutrients and water from soil. However, the mechanisms regulating root growth in hexaploid wheat remain to be elucidated. Here, an integrated comparative proteome study on the roots of two varieties and their descendants with contrasting root phenotypes was performed. A total of 80 differentially expressed proteins (DEPs) associated with the regulation of primary root growth were identified, including two plant steroid biosynthesis related proteins and nine class III peroxidases. Real-time PCR analysis showed that brassinosteroid (BR) biosynthesis pathway was significantly elevated in long-root plants compared with those short-root plants. Moreover, O. and HO were distributed abundantly in both the root meristematic and elongation zones of long root plants, but only in the meristematic zone of short-root plants. The differential distribution of reactive oxygen species (ROS) in the root tips of different genotypes may be caused by the differential expression of peroxidases. Taken together, our results suggest that the regulation of wheat primary root growth is closely related to BR biosynthesis pathway and BR-mediated ROS distribution.
植物根系对于从土壤中获取养分和水分至关重要。然而，六倍体小麦根系生长的调控机制仍有待阐明。在这里，我们对两个品种及其具有不同根系表型的后代的根系进行了综合比较蛋白质组学研究。共鉴定出 80 种与调控主根生长相关的差异表达蛋白（DEPs），包括两种植物甾体生物合成相关蛋白和 9 种 III 类过氧化物酶。实时 PCR 分析表明，长根植株中油菜素内酯（BR）生物合成途径明显高于短根植株。此外，O 和 HO 在长根植株的根分生区和伸长区中均大量分布，但仅在短根植株的分生区中分布。不同基因型根尖中活性氧（ROS）的差异分布可能是由于过氧化物酶的差异表达所致。总之，我们的研究结果表明，小麦主根生长的调控与 BR 生物合成途径和 BR 介导的 ROS 分布密切相关。
