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突触特化支持小脑皮质频率无关的浦肯野细胞输出。

Synaptic Specializations Support Frequency-Independent Purkinje Cell Output from the Cerebellar Cortex.

作者信息

Turecek Josef, Jackman Skyler L, Regehr Wade G

机构信息

Department of Neurobiology, Harvard Medical School, 220 Longwood Ave., Boston, MA 02115, USA.

Department of Neurobiology, Harvard Medical School, 220 Longwood Ave., Boston, MA 02115, USA.

出版信息

Cell Rep. 2016 Dec 20;17(12):3256-3268. doi: 10.1016/j.celrep.2016.11.081.

DOI:10.1016/j.celrep.2016.11.081
PMID:28009294
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5870134/
Abstract

The output of the cerebellar cortex is conveyed to the deep cerebellar nuclei (DCN) by Purkinje cells (PCs). Here, we characterize the properties of the PC-DCN synapse in juvenile and adult mice and find that prolonged high-frequency stimulation leads to steady-state responses that become increasingly frequency independent within the physiological firing range of PCs in older animals, resulting in a linear relationship between charge transfer and activation frequency. We used a low-affinity antagonist to show that GABA-receptor saturation occurs at this synapse but does not underlie frequency-invariant transmission. We propose that PC-DCN synapses have two components of release: one prominent early in trains and another specialized to maintain transmission during prolonged activation. Short-term facilitation offsets partial vesicle depletion to produce frequency-independent transmission.

摘要

小脑皮质的输出通过浦肯野细胞（PCs）传递至小脑深部核团（DCN）。在此，我们描述了幼年和成年小鼠中PC-DCN突触的特性，发现长时间高频刺激会导致稳态反应，在老年动物PC的生理放电范围内，这种反应变得越来越与频率无关，从而在电荷转移和激活频率之间形成线性关系。我们使用一种低亲和力拮抗剂来表明，该突触处发生了GABA受体饱和，但这并不是频率不变性传递的基础。我们提出，PC-DCN突触有两种释放成分：一种在串刺激早期占主导，另一种专门用于在长时间激活期间维持传递。短期易化抵消了部分囊泡耗竭，以产生与频率无关的传递。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2cc6/5870134/4420b2ce37a0/nihms835476f7.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2cc6/5870134/4420b2ce37a0/nihms835476f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2cc6/5870134/994bc19f00c9/nihms835476f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2cc6/5870134/2d99e1aa0823/nihms835476f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2cc6/5870134/b79ffc8fa43c/nihms835476f3.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2cc6/5870134/4420b2ce37a0/nihms835476f7.jpg

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