突触特化支持小脑皮质频率无关的浦肯野细胞输出。
Synaptic Specializations Support Frequency-Independent Purkinje Cell Output from the Cerebellar Cortex.
作者信息
Turecek Josef, Jackman Skyler L, Regehr Wade G
机构信息
Department of Neurobiology, Harvard Medical School, 220 Longwood Ave., Boston, MA 02115, USA.
Department of Neurobiology, Harvard Medical School, 220 Longwood Ave., Boston, MA 02115, USA.
出版信息
Cell Rep. 2016 Dec 20;17(12):3256-3268. doi: 10.1016/j.celrep.2016.11.081.
Abstract
The output of the cerebellar cortex is conveyed to the deep cerebellar nuclei (DCN) by Purkinje cells (PCs). Here, we characterize the properties of the PC-DCN synapse in juvenile and adult mice and find that prolonged high-frequency stimulation leads to steady-state responses that become increasingly frequency independent within the physiological firing range of PCs in older animals, resulting in a linear relationship between charge transfer and activation frequency. We used a low-affinity antagonist to show that GABA-receptor saturation occurs at this synapse but does not underlie frequency-invariant transmission. We propose that PC-DCN synapses have two components of release: one prominent early in trains and another specialized to maintain transmission during prolonged activation. Short-term facilitation offsets partial vesicle depletion to produce frequency-independent transmission.
摘要
小脑皮质的输出通过浦肯野细胞（PCs）传递至小脑深部核团（DCN）。在此，我们描述了幼年和成年小鼠中PC-DCN突触的特性，发现长时间高频刺激会导致稳态反应，在老年动物PC的生理放电范围内，这种反应变得越来越与频率无关，从而在电荷转移和激活频率之间形成线性关系。我们使用一种低亲和力拮抗剂来表明，该突触处发生了GABA受体饱和，但这并不是频率不变性传递的基础。我们提出，PC-DCN突触有两种释放成分：一种在串刺激早期占主导，另一种专门用于在长时间激活期间维持传递。短期易化抵消了部分囊泡耗竭，以产生与频率无关的传递。
相似文献
1
Synaptic Specializations Support Frequency-Independent Purkinje Cell Output from the Cerebellar Cortex.突触特化支持小脑皮质频率无关的浦肯野细胞输出。
Cell Rep. 2016 Dec 20;17(12):3256-3268. doi: 10.1016/j.celrep.2016.11.081.
2
A Slow Short-Term Depression at Purkinje to Deep Cerebellar Nuclear Neuron Synapses Supports Gain-Control and Linear Encoding over Second-Long Time Windows.浦肯野细胞至深部小脑核神经元突触的缓慢短期抑制有助于在 2 秒以上的时间窗口内实现增益控制和线性编码。
J Neurosci. 2020 Jul 29;40(31):5937-5953. doi: 10.1523/JNEUROSCI.2078-19.2020. Epub 2020 Jun 17.
3
Perineuronal Nets in the Deep Cerebellar Nuclei Regulate GABAergic Transmission and Delay Eyeblink Conditioning.深小脑核中的神经周细胞调节 GABA 能传递并延迟眨眼条件反射。
J Neurosci. 2018 Jul 4;38(27):6130-6144. doi: 10.1523/JNEUROSCI.3238-17.2018. Epub 2018 Jun 1.
4
Efficacy and short-term plasticity at GABAergic synapses between Purkinje and cerebellar nuclei neurons.浦肯野细胞与小脑核神经元之间GABA能突触的效能及短期可塑性。
J Neurophysiol. 2003 Feb;89(2):704-15. doi: 10.1152/jn.00558.2002.
5
Synaptogenesis in the cerebellar cortex: differential regulation of gephyrin and GABAA receptors at somatic and dendritic synapses of Purkinje cells.小脑皮质中的突触发生：浦肯野细胞体突触和树突突触处桥连蛋白和GABAA受体的差异调节。
J Comp Neurol. 2008 Jun 1;508(4):579-91. doi: 10.1002/cne.21713.
6
Differential dependence of axo-dendritic and axo-somatic GABAergic synapses on GABAA receptors containing the alpha1 subunit in Purkinje cells.浦肯野细胞中轴突 - 树突状和轴突 - 胞体GABA能突触对含α1亚基的GABAA受体的差异依赖性。
J Neurosci. 2006 Mar 22;26(12):3245-55. doi: 10.1523/JNEUROSCI.5118-05.2006.
7
Unusually Slow Spike Frequency Adaptation in Deep Cerebellar Nuclei Neurons Preserves Linear Transformations on the Subsecond Timescale.小脑深部核神经元异常缓慢的尖峰频率适应在亚秒时间尺度上保持线性变换。
J Neurosci. 2022 Oct 5;42(40):7581-7593. doi: 10.1523/JNEUROSCI.1869-21.2022. Epub 2022 Aug 22.
8
Synaptotagmin 7 confers frequency invariance onto specialized depressing synapses.突触结合蛋白7赋予特殊的抑制性突触频率不变性。
Nature. 2017 Nov 23;551(7681):503-506. doi: 10.1038/nature24474. Epub 2017 Oct 30.
9
Characterization of synaptic connections between cortex and deep nuclei of the rat cerebellum in vitro.大鼠小脑皮质与深部核团之间突触连接的体外特征研究。
Neuroscience. 1995 Feb;64(3):699-712. doi: 10.1016/0306-4522(94)00456-f.
10
Purkinje Cell Collaterals Enable Output Signals from the Cerebellar Cortex to Feed Back to Purkinje Cells and Interneurons.浦肯野细胞侧支使小脑皮质的输出信号能够反馈至浦肯野细胞和中间神经元。
Neuron. 2016 Jul 20;91(2):312-9. doi: 10.1016/j.neuron.2016.05.037. Epub 2016 Jun 23.
引用本文的文献
1
Short-term synaptic dynamics in the ventrolateral and dorsomedial periaqueductal gray.腹外侧和背内侧导水管周围灰质的短期突触动力学
bioRxiv. 2025 Aug 12:2025.08.12.669947. doi: 10.1101/2025.08.12.669947.
2
Computational anatomy: the cerebellar microzone computation.计算解剖学：小脑微区计算
Oxf Open Neurosci. 2025 May 16;4:kvaf001. doi: 10.1093/oons/kvaf001. eCollection 2025.
3
The cerebellar deep nuclei: a patch for rate codes?小脑深部核团：速率编码的一块拼图？
Front Neural Circuits. 2025 Apr 8;19:1548123. doi: 10.3389/fncir.2025.1548123. eCollection 2025.
4
The cerebellum converts input data into a hyper low-resolution granule cell code with spatial dimensions: a hypothesis.小脑将输入数据转换为具有空间维度的超低分辨率颗粒细胞编码：一种假说。
R Soc Open Sci. 2025 Mar 26;12(3):241665. doi: 10.1098/rsos.241665. eCollection 2025 Mar.
5
Distinct release properties of glutamate/GABA co-transmission serve as a frequency-dependent filtering of supramammillary inputs.谷氨酸/γ-氨基丁酸共同传递的独特释放特性可作为乳头体上输入信号的频率依赖性过滤。
Elife. 2024 Dec 16;13:RP99711. doi: 10.7554/eLife.99711.
6
Synaptotagmin-7 Counteracts Short-Term Depression during Phasic Dopamine Release.突触结合蛋白7在阶段性多巴胺释放过程中对抗短期抑郁。
eNeuro. 2024 Mar 12;11(3). doi: 10.1523/ENEURO.0501-23.2024. Print 2024 Mar.
7
Implications of variable synaptic weights for rate and temporal coding of cerebellar outputs.变量突触权重对小脑输出的比率和时间编码的影响。
Elife. 2024 Jan 19;13:e89095. doi: 10.7554/eLife.89095.
8
Interpretation of presynaptic phenotypes of synaptic plasticity in terms of a two-step priming process.根据两步引发过程来解释突触可塑性的突触前表型。
J Gen Physiol. 2024 Jan 1;156(1). doi: 10.1085/jgp.202313454. Epub 2023 Dec 19.
9
A Purkinje cell to parabrachial nucleus pathway enables broad cerebellar influence over the forebrain.浦肯野细胞至臂旁核通路使小脑对前脑产生广泛影响。
Nat Neurosci. 2023 Nov;26(11):1929-1941. doi: 10.1038/s41593-023-01462-w. Epub 2023 Nov 2.
10
Rate versus synchrony codes for cerebellar control of motor behavior.用于运动行为小脑控制的速率与同步代码。
Neuron. 2023 Aug 2;111(15):2448-2460.e6. doi: 10.1016/j.neuron.2023.07.002.
本文引用的文献
1
Purkinje Cell Collaterals Enable Output Signals from the Cerebellar Cortex to Feed Back to Purkinje Cells and Interneurons.浦肯野细胞侧支使小脑皮质的输出信号能够反馈至浦肯野细胞和中间神经元。
Neuron. 2016 Jul 20;91(2):312-9. doi: 10.1016/j.neuron.2016.05.037. Epub 2016 Jun 23.
2
Spontaneous Activity Defines Effective Convergence Ratios in an Inhibitory Circuit.自发活动决定抑制性回路中的有效汇聚比率。
J Neurosci. 2016 Mar 16;36(11):3268-80. doi: 10.1523/JNEUROSCI.3499-15.2016.
3
Determining synaptic parameters using high-frequency activation.使用高频激活来确定突触参数。
J Neurosci Methods. 2016 May 1;264:136-152. doi: 10.1016/j.jneumeth.2016.02.021. Epub 2016 Mar 10.
4
The cerebellum linearly encodes whisker position during voluntary movement.在自主运动过程中，小脑线性编码触须位置。
Elife. 2016 Jan 19;5:e10509. doi: 10.7554/eLife.10509.
5
Nonequivalent release sites govern synaptic depression.不等同的释放位点控制突触抑制。
Proc Natl Acad Sci U S A. 2016 Jan 19;113(3):E378-86. doi: 10.1073/pnas.1523671113. Epub 2015 Dec 29.
6
Climbing fibers encode a temporal-difference prediction error during cerebellar learning in mice.在小鼠小脑学习过程中，攀爬纤维编码时间差异预测误差。
Nat Neurosci. 2015 Dec;18(12):1798-803. doi: 10.1038/nn.4167. Epub 2015 Nov 9.
7
Merits and Limitations of Vesicle Pool Models in View of Heterogeneous Populations of Synaptic Vesicles.囊泡池模型在考虑突触囊泡异质性群体方面的优缺点。
Neuron. 2015 Sep 23;87(6):1131-1142. doi: 10.1016/j.neuron.2015.08.038.
8
Control of inhibitory synaptic outputs by low excitability of axon terminals revealed by direct recording.通过直接记录揭示轴突末梢低兴奋性对抑制性突触输出的控制。
Neuron. 2015 Mar 18;85(6):1273-88. doi: 10.1016/j.neuron.2015.02.013. Epub 2015 Feb 26.
9
Implementation of linear sensory signaling via multiple coordinated mechanisms at central vestibular nerve synapses.通过中枢前庭神经突触处的多种协调机制实现线性感觉信号传递。
Neuron. 2015 Mar 4;85(5):1132-44. doi: 10.1016/j.neuron.2015.01.017. Epub 2015 Feb 19.
10
Short latency cerebellar modulation of the basal ganglia.短潜伏期小脑对基底神经节的调制。
Nat Neurosci. 2014 Dec;17(12):1767-75. doi: 10.1038/nn.3868. Epub 2014 Nov 17.
Zotero 插件