牛至中的酚类化合物及其抗氧化和抗病毒活性。
Phenolic compounds from Origanum vulgare and their antioxidant and antiviral activities.
机构信息
Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University, Guangzhou 51032, China; Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, Jinan University, Guangzhou 51032, China.
School of Life Sciences, The Chinese University of Hong Kong, Hong Kong Special Administrative Region; Food and Nutritional Sciences Programme, The Chinese University of Hong Kong, Hong Kong Special Administrative Region.
出版信息
Food Chem. 2014;152:300-6. doi: 10.1016/j.foodchem.2013.11.153. Epub 2013 Dec 1.
In the present study, six new phenolic compounds (1-6) along with five known ones were isolated from the ethanol extract of the whole plants of Origanum vulgare. The structures of the new compounds were identified on the basis of extensive spectroscopic analyses (UV, IR, NMR, and HRESIMS) and acid hydrolysis. Twenty-one phenolic compounds isolated from O. vulgare in our previous and present studies were evaluated for their in vitro antioxidant activity using 2,2-diphenyl-1-picryhydrazyl (DPPH) radical-scavenging and ferric-reducing antioxidant power (FRAP) assays; twelve of them including two new compounds exhibited significant antioxidant activity comparable to that of ascorbic acid. In addition, the antiviral effects against respiratory syncytial virus (RSV), Coxsackie virus B3 (CVB3) and herpes simplex virus type 1 (HSV-1) were tested by cytopathic effect (CPE) reduction assay.
在本研究中，从牛至全植物的乙醇提取物中分离得到了六种新的酚类化合物（1-6）和五种已知的酚类化合物。新化合物的结构是根据广泛的光谱分析（UV、IR、NMR 和 HRESIMS）和酸水解确定的。从我们之前和现在的研究中从牛至中分离出的 21 种酚类化合物，使用 2,2-二苯基-1-苦基肼（DPPH）自由基清除和铁还原抗氧化能力（FRAP）测定法评估了它们的体外抗氧化活性；其中 12 种包括两种新化合物，表现出与抗坏血酸相当的显著抗氧化活性。此外，通过细胞病变效应（CPE）减少测定法测试了它们对呼吸道合胞病毒（RSV）、柯萨奇病毒 B3（CVB3）和单纯疱疹病毒 1 型（HSV-1）的抗病毒作用。
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