Aydemir Sezer Umran, Ozturk Kevser, Aru Basak, Yanıkkaya Demirel Gulderen, Sezer Serdar, Bozkurt Mehmet Recep
Materials Institute, TUBITAK Marmara Research Center, Kocaeli, 41470, Turkey.
Department of Electrical-Electronic Engineering, Sakarya University, Sakarya, 54187, Turkey.
J Mater Sci Mater Med. 2017 Jan;28(1):19. doi: 10.1007/s10856-016-5831-1. Epub 2016 Dec 23.
Regeneration of nerve, which has limited ability to undergo self-healing, is one of the most challenging areas in the field of tissue engineering. Regarding materials used in neuroregeneration, there is a recent trend toward electrically conductive materials. It has been emphasized that the capacity of conductive materials to regenerate such tissue having limited self-healing ability improves their clinical utility. However, there have been concerns about the safety of materials or fillers used for conductance due to their lack of degradability. Here, we attempt to use poly(Ɛ-caprolactone) (PCL) matrix consisting of varying proportions of zero valent zinc nanoparticles (Zn NPs) via electrospinning. These conductive, biodegradable, and bioactive materials efficiently promoted neuroglial cell proliferation depending on the amount of Zn NPs present in the PCL matrix. Chemical characterizations indicated that the incorporated Zn NPs do not interact with the PCL matrix chemically and that the Zn NPs improved the tensile properties of the PCL matrix. All composites exhibited linear conductivity under in vitro conditions. In vitro cell culture studies were performed to determine the cytotoxicity and proliferative efficiency of materials containing different proportions of Zn NPs. The results were obtained to explore new conductive fillers that can promote tissue regeneration.
神经的再生能力有限，自我修复能力也很弱，这是组织工程领域最具挑战性的领域之一。关于用于神经再生的材料，最近有使用导电材料的趋势。人们强调，具有有限自我修复能力的组织的再生能力提高了导电材料的临床实用性。然而，由于缺乏可降解性，人们对用于传导的材料或填充物的安全性表示担忧。在这里，我们试图通过静电纺丝使用由不同比例的零价锌纳米颗粒（Zn NPs）组成的聚（ε-己内酯）（PCL）基质。这些导电、可生物降解和生物活性材料根据PCL基质中存在的Zn NPs的量有效地促进了神经胶质细胞的增殖。化学表征表明，掺入的Zn NPs与PCL基质没有化学相互作用，并且Zn NPs改善了PCL基质的拉伸性能。所有复合材料在体外条件下均表现出线性导电性。进行了体外细胞培养研究，以确定含有不同比例Zn NPs的材料的细胞毒性和增殖效率。获得这些结果是为了探索能够促进组织再生的新型导电填充物。
