Kiroshka Victoria V, Petrova Valentina A, Chernyakov Daniil D, Bozhkova Yulia O, Kiroshka Katerina V, Baklagina Yulia G, Romanov Dmitry P, Kremnev Roman V, Skorik Yury A
Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Pereyaslavskaya ul. 23, Kharkov, 61015, Ukraine.
Institute of Macromolecular Compounds of the Russian Academy of Sciences, Bolshoi pr. VO 31, St. Petersburg, 199004, Russian Federation.
J Mater Sci Mater Med. 2017 Jan;28(1):21. doi: 10.1007/s10856-016-5822-2. Epub 2016 Dec 23.
Chitosan scaffolds have gained much attention in various tissue engineering applications, but the effect of their microstructure on cell-material spatial interactions remains unclear. Our objective was to evaluate the effect of chitosan-based matrices doping with chitin nano-whiskers (CNW) on adhesion, spreading, cytoskeleton structure, and proliferation of rat bone marrow stromal cells (BMSCs). The behavior of BMSCs during culture on chitosan-CNW films was determined by the molecular mass, hydrophobicity, porosity, crosslinking degree, protonation degree and molecular structure of the composite chitosan-CNW films. The shape, spreading area, cytoskeleton structure, and proliferation of BMSCs on chitosan matrices with a crystalline structure and high porosity were similar to that observed for BMSCs cultured on polystyrene tissue culture plates. The amorphous polymer structure and high swelling led to a decrease in the spreading area and cell proliferation. Thus, we can control the behavior of cells in culture (adhesion, spreading, and proliferation) by changing the physico-chemical properties of the chitosan-CNW films.
壳聚糖支架在各种组织工程应用中备受关注，但其微观结构对细胞与材料空间相互作用的影响仍不明确。我们的目标是评估掺杂几丁质纳米晶须（CNW）的壳聚糖基基质对大鼠骨髓基质细胞（BMSC）的黏附、铺展、细胞骨架结构和增殖的影响。壳聚糖-CNW膜上培养期间BMSC的行为由复合壳聚糖-CNW膜的分子量、疏水性、孔隙率、交联度、质子化程度和分子结构决定。具有晶体结构和高孔隙率的壳聚糖基质上BMSC的形状、铺展面积、细胞骨架结构和增殖与在聚苯乙烯组织培养板上培养的BMSC相似。无定形聚合物结构和高溶胀导致铺展面积和细胞增殖减少。因此，我们可以通过改变壳聚糖-CNW膜的物理化学性质来控制培养中细胞的行为（黏附、铺展和增殖）。
