de Paula Santos-da-Silva Andria, Candido Denise Maria, Nencioni Ana Leonor A, Kimura Louise F, Prezotto-Neto José P, Barbaro Katia Cristina, Chalkidis Hipócrates M, Dorce Valquíria Abrão Coronado
Laboratory of Pharmacology, Butantan Institute, Av. Dr. Vital Brasil, 1500, 05503-900, São Paulo, SP, Brazil.
Laboratory of Arthropods, Butantan Institute, Av. Dr. Vital Brasil, 1500, 05503-900, São Paulo, SP, Brazil.
Toxicon. 2017 Feb;126:51-58. doi: 10.1016/j.toxicon.2016.12.008. Epub 2016 Dec 22.
There are a great number of studies about Brazilian scorpions. However, little is known about the venom of scorpions of northern Brazil, mainly about Tityus obscurus, which is responsible for the most number of accidents in the Amazon. Thus, this study aimed to evaluate some pharmacological effects of T. obscurus venom in rats and mice. In rats, the venom (10 mg/kg i.p.) caused hemorrhagic patches in the lung parenchyma but did not lead to pulmonary edema. There was a decrease in general activity, observed in the activity box after venom injection. The venom did not induce changes in the occurrence and intensity of experimentally induced convulsions, nor did it cause hippocampal neuronal loss. In mice, the LD obtained was 3.13 mg/kg (i.p.). Different doses of the venom (0.2; 1; 5; 10; 15 μg/30 μL per hind paw) induced edematogenic and moderate nociceptive activity in mice. The Tiyus serrulatus venom used as comparison caused more intense symptomatology in mice. Comparing to the venom of other Tityus scorpions of medical importance, that have convulsant and intense nociceptive effects and cause lung edema, as described in the literature, we can conclude that the venom of T. obscurus probably has different characteristics.
有大量关于巴西蝎子的研究。然而，对于巴西北部蝎子的毒液却知之甚少，主要是关于暗纹肥尾蝎，它在亚马逊地区导致的中毒事件最多。因此，本研究旨在评估暗纹肥尾蝎毒液对大鼠和小鼠的一些药理作用。在大鼠中，毒液（10毫克/千克，腹腔注射）导致肺实质出现出血斑，但未导致肺水肿。注射毒液后，在活动箱中观察到大鼠的一般活动减少。毒液并未引起实验性惊厥的发生率和强度发生变化，也未导致海马神经元丢失。在小鼠中，测得的半数致死量为3.13毫克/千克（腹腔注射）。不同剂量的毒液（每只后爪0.2；1；5；10；15微克/30微升）在小鼠中诱导产生致水肿和中度伤害感受活性。用作对照的锯齿肥尾蝎毒液在小鼠中引起更强烈的症状。与文献中描述的具有惊厥和强烈伤害感受作用并导致肺水肿的其他具有医学重要性的肥尾蝎毒液相比，我们可以得出结论，暗纹肥尾蝎的毒液可能具有不同的特性。
