CEB - Centre of Biological Engineering, University of Minho, Campus de Gualtar, Braga 4710-057, Portugal.
CBMA - Center of Molecular and Environmental Biology, University of Minho, Campus de Gualtar, Braga 4710-057, Portugal.
Bioresour Technol. 2017 Mar;227:24-34. doi: 10.1016/j.biortech.2016.12.016. Epub 2016 Dec 11.
In this work, four robust yeast chassis isolated from industrial environments were engineered with the same xylose metabolic pathway. The recombinant strains were physiologically characterized in synthetic xylose and xylose-glucose medium, on non-detoxified hemicellulosic hydrolysates of fast-growing hardwoods (Eucalyptus and Paulownia) and agricultural residues (corn cob and wheat straw) and on Eucalyptus hydrolysate at different temperatures. Results show that the co-consumption of xylose-glucose was dependent on the yeast background. Moreover, heterogeneous results were obtained among different hydrolysates and temperatures for each individual strain pointing to the importance of designing from the very beginning a tailor-made yeast considering the specific raw material and process.
在这项工作中，我们从工业环境中分离出了四个具有鲁棒性的酵母底盘，并在这些底盘中构建了相同的木糖代谢途径。我们在合成木糖和木糖-葡萄糖培养基中、在未经解毒的速生硬木（桉树和泡桐）和农业废弃物（玉米芯和麦秸）的半纤维素水解物以及在不同温度下的桉树水解物中对重组菌株进行了生理特性表征。结果表明，木糖-葡萄糖的共消耗取决于酵母背景。此外，对于每种单独的菌株，不同的水解物和温度之间得到的结果存在差异，这表明从一开始就根据特定的原料和工艺设计定制酵母非常重要。
