School of Information Science & Engineering, Lanzhou University, 222 Tianshui South Road, 730000, China.
School of Information Science & Engineering, Lanzhou University, 222 Tianshui South Road, 730000, China.
Biotechnol Adv. 2017 Mar-Apr;35(2):168-177. doi: 10.1016/j.biotechadv.2016.12.004. Epub 2016 Dec 23.
Optical labels are needed for probing specific target molecules in complex biological systems. As a newly emerging category of tags for molecular imaging in live cells, the Raman label attracts much attention because of the rich information obtained from targeted and untargeted molecules by detecting molecular vibrations. Here, we list three types of Raman probes based on different mechanisms: Surface Enhanced Raman Scattering (SERS) probes, bioorthogonal Raman probes, and Resonance Raman (RR) probes. We review how these Raman probes work for detecting and imaging proteins, nucleic acids, lipids, and other biomolecules in vitro, within cells, or in vivo. We also summarize recent noteworthy studies, expound on the construction of every type of Raman probe and operating principle, sum up in tables typically targeting molecules for specific binding, and provide merits, drawbacks, and future prospects for the three Raman probes.
光学标签用于探测复杂生物系统中的特定靶标分子。作为活细胞中分子成像的新兴标签类别，由于通过检测分子振动可以从靶向和非靶向分子中获得丰富的信息，拉曼标签引起了广泛关注。在这里，我们列出了基于不同机制的三种拉曼探针：表面增强拉曼散射（SERS）探针、生物正交拉曼探针和共振拉曼（RR）探针。我们综述了这些拉曼探针如何在体外、细胞内或体内检测和成像蛋白质、核酸、脂质和其他生物分子。我们还总结了最近的一些值得注意的研究，阐述了每种拉曼探针的构建和工作原理，以表格形式总结了特定结合的典型靶标分子，并对三种拉曼探针的优缺点和未来前景进行了总结。
