Centre for Molecular Nanometrology, WestCHEM, Department of Pure and Applied Chemistry, Technology and Innovation Centre, University of Strathclyde, Glasgow, G1 1RD, United Kingdom; email:
Annu Rev Anal Chem (Palo Alto Calif). 2017 Jun 12;10(1):415-437. doi: 10.1146/annurev-anchem-071015-041557. Epub 2017 Feb 23.
Since its discovery in 1974, surface-enhanced Raman scattering (SERS) has gained momentum as an important tool in analytical chemistry. SERS is used widely for analysis of biological samples, ranging from in vitro cell culture models, to ex vivo tissue and blood samples, and direct in vivo application. New insights have been gained into biochemistry, with an emphasis on biomolecule detection, from small molecules such as glucose and amino acids to larger biomolecules such as DNA, proteins, and lipids. These measurements have increased our understanding of biological systems, and significantly, they have improved diagnostic capabilities. SERS probes display unique advantages in their detection sensitivity and multiplexing capability. We highlight key considerations that are required when performing bioanalytical SERS measurements, including sample preparation, probe selection, instrumental configuration, and data analysis. Some of the key bioanalytical measurements enabled by SERS probes with application to in vitro, ex vivo, and in vivo biological environments are discussed.
自 1974 年发现以来，表面增强拉曼散射（SERS）作为分析化学中的重要工具得到了广泛的关注。SERS 广泛用于生物样本的分析，包括体外细胞培养模型、离体组织和血液样本，以及直接体内应用。人们对生物化学有了新的认识，重点是生物分子的检测，从小分子如葡萄糖和氨基酸到更大的生物分子如 DNA、蛋白质和脂质。这些测量增加了我们对生物系统的理解，并且重要的是，它们提高了诊断能力。SERS 探针在检测灵敏度和多重检测能力方面具有独特的优势。我们强调了在进行生物分析 SERS 测量时需要考虑的关键因素，包括样品制备、探针选择、仪器配置和数据分析。讨论了一些通过 SERS 探针实现的关键生物分析测量，这些探针适用于体外、离体和体内生物环境。
