Soto Karen M, Hernández-Iturriaga Montserrat, Loarca-Piña Guadalupe, Luna-Bárcenas Gabriel, Gómez-Aldapa Carlos A, Mendoza Sandra
Programa de Posgrado en Alimentos del Centro de la República (PROPAC), Research and Graduate Studies in Food Science, School of Chemistry, Universidad Autónoma de Querétaro, 76010 Querétaro, Qro Mexico.
Centro de investigación y de estudios avanzados del IPN, Cinvestav, Querétaro, Mexico.
J Food Sci Technol. 2016 Oct;53(10):3787-3794. doi: 10.1007/s13197-016-2365-y. Epub 2016 Oct 31.
Most of antimicrobial peptides interact with food components decreasing their activity, which limit their successful incorporation into packaging material, functional foods and edible films. The aim of this work was to develop a nisin carrier. Nanofibers of amaranth protein and pullulan (50:50) loaded with nisin were obtained by electrospinning. The nanofibers morphology was determined by scanning electron microscopy and fluorescent microscopy. The molecular interactions were characterized by infrared spectroscopy, X-ray diffraction, differential scanning calorimetry, and thermogravimetric analysis. The nisin loading efficiency as well as the antimicrobial activity against were evaluated. The micrographs of the obtained materials exhibited smooth and continuous fibers with no defects characterized by diameters between 124 and 173 nm. The FTIR analysis showed intermolecular interactions mainly by hydrogen bonding. The electrospinning process improved the thermal properties of the polymeric mixture displacing the Tm peak to higher temperatures and increasing crystallinity. The antimicrobial activity of nisin in broth and agar against was maintained after incorporation into fibers. The results presented an outlook for the potential use of protein amaranth nanofibers when incorporating antimicrobials as a food preservation strategy.
大多数抗菌肽会与食品成分相互作用，降低其活性，这限制了它们成功应用于包装材料、功能食品和可食用薄膜中。这项工作的目的是开发一种乳酸链球菌素载体。通过静电纺丝获得了负载乳酸链球菌素的苋菜蛋白和普鲁兰多糖（50:50）纳米纤维。通过扫描电子显微镜和荧光显微镜确定纳米纤维的形态。通过红外光谱、X射线衍射、差示扫描量热法和热重分析对分子间相互作用进行表征。评估了乳酸链球菌素的负载效率以及对……的抗菌活性。所获得材料的显微照片显示出光滑连续、无缺陷的纤维，直径在124至173纳米之间。傅里叶变换红外光谱分析表明分子间相互作用主要通过氢键进行。静电纺丝过程改善了聚合物混合物的热性能，使熔点峰向更高温度移动并提高了结晶度。将乳酸链球菌素掺入纤维后，其在肉汤和琼脂中对……的抗菌活性得以保持。研究结果为将苋菜蛋白纳米纤维作为一种食品保鲜策略来负载抗菌剂的潜在应用提供了前景。
