Vanhoe H, Vandecasteele C, Versieck J, Dams R
Anal Chem. 1989 Sep 1;61(17):1851-7. doi: 10.1021/ac00192a014.
A method was developed for the determination of seven trace elements (Fe, Co, Cu, Zn, Rb, Mo, and Cs) in human serum by inductively coupled plasma mass spectrometry (ICP-MS). Sample preparation was kept as limited as possible. As the only sample pretreatment serum samples were diluted with nitric acid and indium was added as an internal standard. The results for iron, cobalt, copper, and zinc were corrected for interferences from polyatomic ions by using a blank solution containing the same concentration of sodium, sulfur, chlorine, and calcium as human serum. For copper and zinc the corrections are small, whereas for iron and cobalt they are important. No interferences occur for the considered isotopes of rubidium, molybdenum, and cesium. In order to test the accuracy and precision of the analytical technique, a "second-generation biological reference material (human serum)" was analyzed. The results obtained by ICP-MS for the seven elements considered showed good agreement with the certified values.
开发了一种通过电感耦合等离子体质谱法（ICP-MS）测定人血清中七种微量元素（铁、钴、铜、锌、铷、钼和铯）的方法。样品制备尽可能简化。作为唯一的样品预处理，血清样品用硝酸稀释，并加入铟作为内标。通过使用含有与人类血清相同浓度的钠、硫、氯和钙的空白溶液，校正了铁、钴、铜和锌因多原子离子产生的干扰。对于铜和锌，校正量较小，而对于铁和钴，校正量则很重要。对于所考虑的铷、钼和铯的同位素，未出现干扰。为了测试分析技术的准确性和精密度，对一种“第二代生物参考物质（人血清）”进行了分析。通过ICP-MS获得的所考虑的七种元素的结果与认证值显示出良好的一致性。
