Research Department of Zoology, VHNSN College (Autonomous), Virudhunagar, Tamilnadu, 626001, India.
Department of Zoology, PSG College of Arts and Science, Coimbatore- 14, Tamilnadu, 626001, India.
Carbohydr Polym. 2017 Feb 20;158:141-148. doi: 10.1016/j.carbpol.2016.11.083. Epub 2016 Nov 29.
In the present study silver nanoparticles were synthesized by reduction of AgNO using aqueous CMC solution, which acts as both reducing and capping agent. The formation of AgNO nanoparticles was observed visually by color change and these nanoparticles were characterized through UV-vis spectroscopy, FTIR, XRD, SEM, EDS and AFM. The FTIR peaks observed to be ranging from 3300 to 605cm. The AFM image clearly showed the surface morphology of well dispersed nanoparticles. SEM image illustrates the nanoparticles with spherical shape. The crystalline nature of the particles was assured by XRD analysis. The antimicrobial activity of nanoparticles was tested against human bacterial pathogens (Bacillus cereus, Staphylococcus aureus, S. epidermidis, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella typhimurium &Vibrio vulnificus). The bacterial growth was highly inhibited by the nanoparticles. The synergistic effect of nanoparticles in combination with selected broad spectrum antibiotics against the tested bacteria determined strong growth inhibitory activity.
在本研究中，通过用 CMC 水溶液还原 AgNO3 来合成银纳米粒子，该 CMC 水溶液同时起到还原剂和稳定剂的作用。AgNO3 纳米粒子的形成通过颜色变化进行直观观察，并用紫外可见分光光度计、傅里叶变换红外光谱、X 射线衍射、扫描电子显微镜、能谱仪和原子力显微镜进行了表征。FTIR 峰观察到的范围从 3300 到 605cm。原子力显微镜图像清楚地显示了分散良好的纳米粒子的表面形态。扫描电子显微镜图像说明了纳米粒子具有球形形状。通过 X 射线衍射分析确定了颗粒的结晶性质。用纳米粒子测试了对人类细菌病原体（蜡样芽孢杆菌、金黄色葡萄球菌、表皮葡萄球菌、大肠杆菌、肺炎克雷伯菌、铜绿假单胞菌、鼠伤寒沙门氏菌和创伤弧菌）的抗菌活性。纳米粒子对细菌的生长有很强的抑制作用。纳米粒子与选定的广谱抗生素联合使用对测试细菌的协同作用表现出很强的生长抑制活性。
